BaBao Dan Suppresses Tumor Growth of Pancreatic Cancer Through Modulating Transcriptional Reprogramming of Cancer-Related Genes

Libin Song; Libin Song; Zhixiao Fang; Chuanfang Pan; Xiangyuan Chen; Xiang Qian; Xiang Qian; Yunyun Cai; Xiumei Zhang; Xiumei Zhang; Luming Liu; Luming Liu

doi:10.3389/fonc.2020.584330

Frontiers in Oncology (Nov 2020)

BaBao Dan Suppresses Tumor Growth of Pancreatic Cancer Through Modulating Transcriptional Reprogramming of Cancer-Related Genes

Libin Song,
Libin Song,
Zhixiao Fang,
Chuanfang Pan,
Xiangyuan Chen,
Xiang Qian,
Xiang Qian,
Yunyun Cai,
Xiumei Zhang,
Xiumei Zhang,
Luming Liu,
Luming Liu

Affiliations

Libin Song: Department of Integrative Oncology, Fudan University, Shanghai Cancer Center, Shanghai, China
Libin Song: Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China
Zhixiao Fang: Institute of Translational Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Chuanfang Pan: Department of Oncology, Longhua Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China
Xiangyuan Chen: Department of Geriatrics, Huadong Hospital, Shanghai Medical College, Fudan University, Shanghai, China
Xiang Qian: Institute of Cancer and Basic Medicine (ICBM), Chinese Academy of Sciences, Zheijang, China
Xiang Qian: Department of Traditional Chinese Medicine, Zhejiang Cancer Hospitall, Zheijang, China
Yunyun Cai: Department of Integrated Traditional Western Medicine, Minhang Branch of Fudan University of Shanghai Cancer Center, Shanghai, China
Xiumei Zhang: Department of Integrative Oncology, Fudan University, Shanghai Cancer Center, Shanghai, China
Xiumei Zhang: Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China
Luming Liu: Department of Integrative Oncology, Fudan University, Shanghai Cancer Center, Shanghai, China
Luming Liu: Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China

DOI: https://doi.org/10.3389/fonc.2020.584330
Journal volume & issue: Vol. 10

Abstract

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Background and AimsPancreatic ductal adenocarcinoma (PDAC) is one of refractory malignancies without efficient therapeutics. Babao Dan (BBD) was partially effective to suppress tumor growth of PDAC in clinical practice. However, the molecular mechanisms were unclear.MethodsWe established PDAC mice models and treated them with BBD through intragastric administration. Treatment and control groups were then subjected to high-throughput RNA sequencing. We presented the transcriptional changes upon BBD treatment by using computational analysis comparing BBD treatment and control groups. Functional enrichment analysis was employed to investigate the biological processes or pathways that BBD modulates.ResultsBBD treatment showed strong suppression on tumor growth of PDAC, even stronger than Gemcitabine. Through differential analysis comparing BBD treatment and control groups, we identified 638 up-regulated and 259 down-regulated genes in the BBD treatment group. BBD was found to activate tumor suppressor genes, such as MTUS1, PDGFB, SOD3, and UCHL1. Furthermore, we revealed that BBD treatment inhibited cancer-related pathways and elevated activities of metabolism-related processes. The BBD-modulated metabolic genes were further showed to be associated with patient survival in an independent cohort with pancreatic cancer.ConclusionBBD repressed the tumor growth of PDAC. BBD treatment modulated expression of cancer-related genes in PDAC. BBD suppressed cancer-related pathways and activated metabolic processes in PDAC. Our study suggests BBD treatment as potential effective therapeutics for patients with pancreatic cancer.

Published in Frontiers in Oncology

ISSN: 2234-943X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Website: https://www.frontiersin.org/journals/oncology/

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