A genetically encoded fluorescent biosensor for detecting itaconate with subcellular resolution in living macrophages

Pengkai Sun; Zhenxing Zhang; Bin Wang; Caiyun Liu; Chao Chen; Ping Liu; Xinjian Li

doi:10.1038/s41467-022-34306-5

Nature Communications (Nov 2022)

A genetically encoded fluorescent biosensor for detecting itaconate with subcellular resolution in living macrophages

Pengkai Sun,
Zhenxing Zhang,
Bin Wang,
Caiyun Liu,
Chao Chen,
Ping Liu,
Xinjian Li

Affiliations

Pengkai Sun: CAS Key Laboratory of Infection and Immunity, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
Zhenxing Zhang: CAS Key Laboratory of Infection and Immunity, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
Bin Wang: CAS Key Laboratory of Infection and Immunity, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
Caiyun Liu: CAS Key Laboratory of Infection and Immunity, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
Chao Chen: CAS Key Laboratory of Infection and Immunity, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
Ping Liu: CAS Key Laboratory of Infection and Immunity, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
Xinjian Li: CAS Key Laboratory of Infection and Immunity, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences

DOI: https://doi.org/10.1038/s41467-022-34306-5
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 12

Abstract

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Itaconate has been identified as an immunomodulatory metabolite produced by activated macrophages, but methods for detecting itaconate in live cells are lacking. Here, the authors develop a fluorescent biosensor named BioITA for detecting itaconate in subcellular compartments of living macrophages.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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