Cancer Management and Research (Jun 2021)
Hsa_circRNA_000543 Predicts Poor Prognosis and Promotes Cervical Cancer Cell Progression Through Regulating miR-567/ZNF268 Axis
Abstract
Zhilan Yao,1,2 Liuping Shu,1,2 Yi Yi,1,2 Lifu Qiao1,2 1Department of Gynecology, Wujin Hospital Affiliated with Jiangsu University, Changzhou, Jiangsu Province, 213100, People’s Republic of China; 2Department of Gynecology, The Wujin Clinical College of Xuzhou Medical University, Changzhou, Jiangsu Province, 213100, People’s Republic of ChinaCorrespondence: Yi Yi; Lifu QiaoDepartment of Gynecology, Wujin Hospital Affiliated with Jiangsu University, No. 2 Yongning North Road, Changzhou, Jiangsu Province, 213100, People’s Republic of ChinaTel +86-0519-85336190Email [email protected]; [email protected]: Cervical cancer (CC) is the fourth most common cancer among women worldwide. We aimed to explore the role of hsa_circ_000543 played in CC.Methods: The hsa_circ_000543 expressions in CC tissues and cells were measured by qRT-PCR. The correlation of hsa_circ_000543 expression and the clinical features of CC patients were analyzed by SPSS 20.0. The up- or down-regulated plasmids of hsa_circ_000543 were respectively transfected into CC cells. Cell proliferation, apoptosis and colony formation were detected through CCK-8 assay, flow cytometry and cell colony formation assay, respectively. The cell migration and invasion were evaluated by Transwell assay. The underlying molecular mechanism of hsa_circ_000543 was studied by bioinformatic prediction tools and luciferase reporter assay. Rescue experiments were performed to validate the regulation mechanism of hsa_circ_000543/miR-567/ZNF268 axis in CC.Results: Hsa_circ_000543 was over-expressed in CC tissues and cells. The high expression of hsa_circ_000543 indicated poor prognosis of CC patients. Hsa_circ_000543 promoted cell proliferation, colony formation, migration and invasion, as well as inhibited cell apoptosis in CC cells. Hsa_circ_000543 directly targeted miR-567/ZNF268 in CC cell lines. In CC tumor tissues and cells, the hsa_circ_000543 expression was negatively correlated with miR-567 expression and showed a positive correlation with ZNF268 expression. The rescue experiments revealed that hsa_circ_000543 mediated cell proliferation, apoptosis, colony formation, migration and invasion of CC cells via regulating miR-567/ZNF268 axis.Conclusion: Hsa_circ_000543 regulated CC cell activities through binding miR-567 and therefore enhancing ZNF268 expression.Keywords: cervical cancer, circular RNA, hsa_circ_000543, miR-567, ZNF268
