Frontiers in Immunology (Jan 2018)
Minimum Information about T Regulatory Cells: A Step toward Reproducibility and Standardization
- Anke Fuchs,
- Mateusz Gliwiński,
- Nathali Grageda,
- Rachel Spiering,
- Abul K. Abbas,
- Silke Appel,
- Rosa Bacchetta,
- Manuela Battaglia,
- David Berglund,
- Bruce Blazar,
- Jeffrey A. Bluestone,
- Martin Bornhäuser,
- Anja ten Brinke,
- Todd M. Brusko,
- Nathalie Cools,
- Maria Cristina Cuturi,
- Edward Geissler,
- Nick Giannoukakis,
- Karolina Gołab,
- David A. Hafler,
- S. Marieke van Ham,
- Joanna Hester,
- Keli Hippen,
- Mauro Di Ianni,
- Natasa Ilic,
- John Isaacs,
- John Isaacs,
- Fadi Issa,
- Dorota Iwaszkiewicz-Grześ,
- Elmar Jaeckel,
- Irma Joosten,
- David Klatzmann,
- Hans Koenen,
- Cees van Kooten,
- Olle Korsgren,
- Olle Korsgren,
- Karsten Kretschmer,
- Karsten Kretschmer,
- Megan Levings,
- Natalia Maria Marek-Trzonkowska,
- Marc Martinez-Llordella,
- Djordje Miljkovic,
- Kingston H.G. Mills,
- Joana P. Miranda,
- Ciriaco A. Piccirillo,
- Amy L. Putnam,
- Thomas Ritter,
- Maria Grazia Roncarolo,
- Shimon Sakaguchi,
- Silvia Sánchez-Ramón,
- Birgit Sawitzki,
- Ljiljana Sofronic-Milosavljevic,
- Megan Sykes,
- Qizhi Tang,
- Marta Vives-Pi,
- Herman Waldmann,
- Piotr Witkowski,
- Kathryn J. Wood,
- Silvia Gregori,
- Catharien M. U. Hilkens,
- Giovanna Lombardi,
- Phillip Lord,
- Eva M. Martinez-Caceres,
- Piotr Trzonkowski
Affiliations
- Anke Fuchs
- GMP facility, DFG-Center for Regenerative Therapies Dresden (CRTD), Center for Molecular and Cellular Bioengineering (CMCB), and Department of Internal Medicine I, University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany
- Mateusz Gliwiński
- Department of Clinical Immunology and Transplantology, Medical University of Gdańsk, Gdańsk, Poland
- Nathali Grageda
- MRC Centre for Transplantation, King’s College London, Guy’s Hospital, London, United Kingdom
- Rachel Spiering
- Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, United Kingdom
- Abul K. Abbas
- Department of Pathology, University of California, San Francisco, San Francisco, CA, United States
- Silke Appel
- Broegelmann Research Laboratory, Department of Clinical Science, University of Bergen, Bergen, Norway
- Rosa Bacchetta
- Pediatric Stem Cell Transplantation and Regenerative Medicine, Department of Pediatrics, Stanford School of Medicine, Stanford, CA, United States
- Manuela Battaglia
- Diabetes Research Institute, IRCCS San Raffaele Scientific Institute, and TrialNet Clinical Center, San Raffaele Hospital, Milan, Italy
- David Berglund
- Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
- Bruce Blazar
- 0Department of Pediatrics, Division of Blood and Marrow Transplantation, University of Minnesota, Minnesota, MN, United States
- Jeffrey A. Bluestone
- 1Hormone Research Institute, University of California, San Francisco, San Francisco, CA, United States
- Martin Bornhäuser
- GMP facility, DFG-Center for Regenerative Therapies Dresden (CRTD), Center for Molecular and Cellular Bioengineering (CMCB), and Department of Internal Medicine I, University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany
- Anja ten Brinke
- 2Department of Immunopathology, Sanquin Research and Landsteiner Laboratory, University of Amsterdam, Academic Medical Center, Amsterdam, Netherlands
- Todd M. Brusko
- 3Department of Pathology, Immunology, and Laboratory Medicine, University of Florida Diabetes Institute, College of Medicine, Gainesville, FL, United States
- Nathalie Cools
- 4Laboratory of Experimental Hematology, Vaccine & Infectious Disease Institute, Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp University Hospital (UZA), Edegem, Belgium
- Maria Cristina Cuturi
- 5Centre de Recherche en Transplantation et Immunologie UMR1064, INSERM, Université de Nantes, Nantes, France
- Edward Geissler
- 6Division of Experimental Surgery, Department of Surgery, University Hospital Regensburg, Regensburg, Germany
- Nick Giannoukakis
- 7Allegheny Health Network, Institute of Cellular Therapeutics, Carnegie Mellon University, Pittsburgh, PA, United States
- Karolina Gołab
- 8Transplant Institute, Department of Surgery, The University of Chicago, Chicago, IL, United States
- David A. Hafler
- 9Departments of Neurology and Immunobiology, Yale School of Medicine, New Haven, CT, United States
- S. Marieke van Ham
- 2Department of Immunopathology, Sanquin Research and Landsteiner Laboratory, University of Amsterdam, Academic Medical Center, Amsterdam, Netherlands
- Joanna Hester
- 0Nuffield Department of Surgical Sciences, John Radcliffe Hospital, University of Oxford, Oxford, United Kingdom
- Keli Hippen
- 0Department of Pediatrics, Division of Blood and Marrow Transplantation, University of Minnesota, Minnesota, MN, United States
- Mauro Di Ianni
- 1Department of Medicine and Aging Sciences, University of Chieti-Pescara, Chieti, Italy
- Natasa Ilic
- 2Department for Immunology and Immunoparasitology, National Reference Laboratory for Trichinellosis, Institute for the Application of Nuclear Energy, University of Belgrade, Belgrade, Serbia
- John Isaacs
- Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, United Kingdom
- John Isaacs
- 3National Institute for Health Research Newcastle Biomedical Research Centre at Newcastle upon Tyne Hospitals NHS Foundation Trust and Newcastle University, Newcastle upon Tyne, United Kingdom
- Fadi Issa
- 0Nuffield Department of Surgical Sciences, John Radcliffe Hospital, University of Oxford, Oxford, United Kingdom
- Dorota Iwaszkiewicz-Grześ
- Department of Clinical Immunology and Transplantology, Medical University of Gdańsk, Gdańsk, Poland
- Elmar Jaeckel
- 4Department of Gastroenterology, Hepatology, Endocrinology, Diabetology, Transplantationsforschungszentrum, Medical School of Hannover (MHH), Hannover, Germany
- Irma Joosten
- 5Laboratory of Medical Immunology, Department of Laboratory Medicine, Radboudumc, Nijmegen, Netherlands
- David Klatzmann
- 6Immunology-Immunopathology-Immunotherapy (i3), UPMC Univ Paris 06, UMRS 959, Sorbonne Université, and Biotherapy (CIC-BTi) and Inflammation-Immunopathology-Biotherapy Department, AP-HP, Hôpital Pitié-Salpêtrière, Paris, France
- Hans Koenen
- 5Laboratory of Medical Immunology, Department of Laboratory Medicine, Radboudumc, Nijmegen, Netherlands
- Cees van Kooten
- 7Department of Nephrology, Leiden University Medical Center, Leiden, Netherlands
- Olle Korsgren
- 8Department of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University Hospital, Uppsala, Sweden
- Olle Korsgren
- 9Transplantation Immunology, Gothenburg University, Gothenburg, Sweden
- Karsten Kretschmer
- 0Molecular and Cellular Immunology/Immune Regulation, DFG-Center for Regenerative Therapies Dresden (CRTD), Center for Molecular and Cellular Bioengineering (CMCB), Technische Universität Dresden, and Paul Langerhans Institute Dresden (PLID) of the Helmholtz Zentrum München at the University Hospital and Medical Faculty Carl Gustav Carus of TU Dresden, Dresden, Germany
- Karsten Kretschmer
- 1German Center for Diabetes Research (DZD e.V.), Neuherberg, Germany
- Megan Levings
- 2Department of Surgery, Faculty of Medicine, The University of British Columbia, BC Children’s Hospital Research Institute, Vancouver, BC, Canada
- Natalia Maria Marek-Trzonkowska
- 3Laboratory of Immunoregulation and Cellular Therapies, Department of Family Medicine, Medical University of Gdańsk, Gdańsk, Poland
- Marc Martinez-Llordella
- 4Medical Research Council Centre for Transplantation, Institute of Liver Studies, King’s College London, London, United Kingdom
- Djordje Miljkovic
- 5Department of Immunology, IBISS, University of Belgrade, Belgrade, Serbia
- Kingston H.G. Mills
- 6Immune Regulation Research Group, School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Ireland
- Joana P. Miranda
- 7Faculty of Pharmacy, Research Institute for Medicines (iMed.ULisboa), Universidade de Lisboa, Lisbon, Portugal
- Ciriaco A. Piccirillo
- 8Departments of Microbiology & Immunology and Medicine, Faculty of Medicine, McGill University, Program in Infectious Disease and Immunity in Global Health, Centre of Excellence in Translational Immunology (CETI), Research Institute of McGill University Health Centre, Montréal, QC, Canada
- Amy L. Putnam
- 1Hormone Research Institute, University of California, San Francisco, San Francisco, CA, United States
- Thomas Ritter
- 9College of Medicine, Nursing and Health Sciences, Regenerative Medicine Institute (REMEDI), Biomedical Sciences, National University of Ireland, Galway, Ireland
- Maria Grazia Roncarolo
- 0Division of Stem Cell Transplantation and Regenerative Medicine, Department of Pediatrics, ISCBRM, Stanford School of Medicine, Stanford, CA, United States
- Shimon Sakaguchi
- 1WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan
- Silvia Sánchez-Ramón
- 2Department of Clinical Immunology, Hospital Clínico San Carlos, Universidad Complutense of Madrid, Madrid, Spain
- Birgit Sawitzki
- 3Institute for Medical Immunology, Charité – Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health, Berlin, Germany
- Ljiljana Sofronic-Milosavljevic
- 2Department for Immunology and Immunoparasitology, National Reference Laboratory for Trichinellosis, Institute for the Application of Nuclear Energy, University of Belgrade, Belgrade, Serbia
- Megan Sykes
- 4Columbia Center for Translational Immunology, Columbia University College of Physicians and Surgeons, Bone Marrow Transplantation Research, Division of Hematology/Oncology, Columbia University Medical Center, Columbia University, New York, NY, United States
- Qizhi Tang
- 5Department of Surgery, University of California, San Francisco, San Francisco, CA, United States
- Marta Vives-Pi
- 6Immunology of Diabetes Unit, Germans Trias i Pujol Research Institute (IGTP), Barcelona, Spain
- Herman Waldmann
- 7Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom
- Piotr Witkowski
- 8Transplant Institute, Department of Surgery, The University of Chicago, Chicago, IL, United States
- Kathryn J. Wood
- 0Nuffield Department of Surgical Sciences, John Radcliffe Hospital, University of Oxford, Oxford, United Kingdom
- Silvia Gregori
- 8Mechanisms of Peripheral Tolerance Group, San Raffaele Telethon Institute for Gene Therapy (SR-TIGET), San Raffaele Scientific Institute IRCCS, Milan, Italy
- Catharien M. U. Hilkens
- Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, United Kingdom
- Giovanna Lombardi
- MRC Centre for Transplantation, King’s College London, Guy’s Hospital, London, United Kingdom
- Phillip Lord
- 9School of Computing, Newcastle University, Newcastle upon Tyne, United Kingdom
- Eva M. Martinez-Caceres
- 0Immunology Division, Germans Trias i Pujol University Hospital - Can Ruti, Department Cellular Biology, Physiology, Immunology, Universitat Autònoma Barcelona, Badalona, Spain
- Piotr Trzonkowski
- Department of Clinical Immunology and Transplantology, Medical University of Gdańsk, Gdańsk, Poland
- DOI
- https://doi.org/10.3389/fimmu.2017.01844
- Journal volume & issue
-
Vol. 8
Abstract
Cellular therapies with CD4+ T regulatory cells (Tregs) hold promise of efficacious treatment for the variety of autoimmune and allergic diseases as well as posttransplant complications. Nevertheless, current manufacturing of Tregs as a cellular medicinal product varies between different laboratories, which in turn hampers precise comparisons of the results between the studies performed. While the number of clinical trials testing Tregs is already substantial, it seems to be crucial to provide some standardized characteristics of Treg products in order to minimize the problem. We have previously developed reporting guidelines called minimum information about tolerogenic antigen-presenting cells, which allows the comparison between different preparations of tolerance-inducing antigen-presenting cells. Having this experience, here we describe another minimum information about Tregs (MITREG). It is important to note that MITREG does not dictate how investigators should generate or characterize Tregs, but it does require investigators to report their Treg data in a consistent and transparent manner. We hope this will, therefore, be a useful tool facilitating standardized reporting on the manufacturing of Tregs, either for research purposes or for clinical application. This way MITREG might also be an important step toward more standardized and reproducible testing of the Tregs preparations in clinical applications.
Keywords
- minimum information model
- T regulatory cells
- immunotherapy
- good manufacturing practice
- cell therapy
- immune tolerance
