Nature Communications (Sep 2021)
In vivo inducible reverse genetics in patients’ tumors to identify individual therapeutic targets
- Michela Carlet,
- Kerstin Völse,
- Jenny Vergalli,
- Martin Becker,
- Tobias Herold,
- Anja Arner,
- Daniela Senft,
- Vindi Jurinovic,
- Wen-Hsin Liu,
- Yuqiao Gao,
- Veronika Dill,
- Boris Fehse,
- Claudia D. Baldus,
- Lorenz Bastian,
- Lennart Lenk,
- Denis M. Schewe,
- Johannes W. Bagnoli,
- Binje Vick,
- Jan Philipp Schmid,
- Alexander Wilhelm,
- Rolf Marschalek,
- Philipp J. Jost,
- Cornelius Miething,
- Kristoffer Riecken,
- Marc Schmidt-Supprian,
- Vera Binder,
- Irmela Jeremias
Affiliations
- Michela Carlet
- Research Unit Apoptosis in Hematopoietic Stem Cells, Helmholtz Zentrum München, German Research Center for Environmental Health (HMGU)
- Kerstin Völse
- Research Unit Apoptosis in Hematopoietic Stem Cells, Helmholtz Zentrum München, German Research Center for Environmental Health (HMGU)
- Jenny Vergalli
- Research Unit Apoptosis in Hematopoietic Stem Cells, Helmholtz Zentrum München, German Research Center for Environmental Health (HMGU)
- Martin Becker
- Research Unit Apoptosis in Hematopoietic Stem Cells, Helmholtz Zentrum München, German Research Center for Environmental Health (HMGU)
- Tobias Herold
- Research Unit Apoptosis in Hematopoietic Stem Cells, Helmholtz Zentrum München, German Research Center for Environmental Health (HMGU)
- Anja Arner
- Department of Pediatrics, Dr. von Hauner Children’s Hospital, University Hospital, Ludwig Maximilian University (LMU)
- Daniela Senft
- Research Unit Apoptosis in Hematopoietic Stem Cells, Helmholtz Zentrum München, German Research Center for Environmental Health (HMGU)
- Vindi Jurinovic
- Research Unit Apoptosis in Hematopoietic Stem Cells, Helmholtz Zentrum München, German Research Center for Environmental Health (HMGU)
- Wen-Hsin Liu
- Research Unit Apoptosis in Hematopoietic Stem Cells, Helmholtz Zentrum München, German Research Center for Environmental Health (HMGU)
- Yuqiao Gao
- Research Unit Apoptosis in Hematopoietic Stem Cells, Helmholtz Zentrum München, German Research Center for Environmental Health (HMGU)
- Veronika Dill
- Clinic and Policlinic for Internal Medicine III, Technical University of Munich, School of Medicine
- Boris Fehse
- Research Department Cell and Gene Therapy, Department of Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf
- Claudia D. Baldus
- Internal Medicine II, Christian-Albrechts University Kiel and University Medical Center Schleswig-Holstein, Campus Kiel
- Lorenz Bastian
- Internal Medicine II, Christian-Albrechts University Kiel and University Medical Center Schleswig-Holstein, Campus Kiel
- Lennart Lenk
- Department of Pediatrics I, ALL-BFM Study Group, Christian-Albrechts University Kiel and University Medical Center Schleswig-Holstein
- Denis M. Schewe
- Department of Pediatrics I, ALL-BFM Study Group, Christian-Albrechts University Kiel and University Medical Center Schleswig-Holstein
- Johannes W. Bagnoli
- Anthropology and Human Genomics, Faculty of Biology, Ludwig Maximilian University (LMU)
- Binje Vick
- Research Unit Apoptosis in Hematopoietic Stem Cells, Helmholtz Zentrum München, German Research Center for Environmental Health (HMGU)
- Jan Philipp Schmid
- Research Unit Apoptosis in Hematopoietic Stem Cells, Helmholtz Zentrum München, German Research Center for Environmental Health (HMGU)
- Alexander Wilhelm
- Institute of Pharmaceutical Biology, Diagnosic Center of Acute Leukemias (DCAL), Goethe-University
- Rolf Marschalek
- Institute of Pharmaceutical Biology, Diagnosic Center of Acute Leukemias (DCAL), Goethe-University
- Philipp J. Jost
- German Cancer Consortium (DKTK), Partnering Site Munich
- Cornelius Miething
- Department of Medicine I, Medical Center, University of Freiburg, Faculty of Medicine
- Kristoffer Riecken
- Research Department Cell and Gene Therapy, Department of Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf
- Marc Schmidt-Supprian
- Institute of Experimental Hematology, Technical University of Munich
- Vera Binder
- Department of Pediatrics, Dr. von Hauner Children’s Hospital, University Hospital, Ludwig Maximilian University (LMU)
- Irmela Jeremias
- Research Unit Apoptosis in Hematopoietic Stem Cells, Helmholtz Zentrum München, German Research Center for Environmental Health (HMGU)
- DOI
- https://doi.org/10.1038/s41467-021-25963-z
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 11
Abstract
Preclinical molecular models are useful that mimic a patient´s response to targeted therapy. Here, the authors establish an in vivo inducible RNAi-mediated gene silencing system in patient-derived xenograft models of acute leukemia to identify individual vulnerabilities and therapeutic targets.
