Nature Communications (Apr 2020)

Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair

  • Beeke Wienert,
  • David N. Nguyen,
  • Alexis Guenther,
  • Sharon J. Feng,
  • Melissa N. Locke,
  • Stacia K. Wyman,
  • Jiyung Shin,
  • Katelynn R. Kazane,
  • Georgia L. Gregory,
  • Matthew A. M. Carter,
  • Francis Wright,
  • Bruce R. Conklin,
  • Alex Marson,
  • Chris D. Richardson,
  • Jacob E. Corn

DOI
https://doi.org/10.1038/s41467-020-15845-1
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 15

Abstract

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Altering cellular responses to double-strand breaks in DNA could rebalance CRISPRediting outcomes. Here, the authors use a pooled CRISPR screen to identify inhibition of CDC7 as a strategy to improve HDR outcomes.