Comparison of three types of anaerobic granular sludge for treating pharmaceutical wastewater

Abstract

Three types of anaerobic granular sludge for treating chemical synthesis-based pharmaceutical wastewater were compared: (1) an up-flow anaerobic sludge blanket (UASB) filled with polyvinyl alcohol (PVA) gel beads (UASB-PVA); (2) a UASB filled with traditional anaerobic granular sludge; and (3) a UASB filled with traditional anaerobic granular sludge and granular active carbon (UASB-GAC). The domestication times for the UASB-PVA, UASB, and UASB-GAC reactors were 30, 47, and 47 days, respectively. When the organic loading rate (OLR) was up to 7 kg chemical oxygen demand (COD)/(m3·d), the COD efficiency of the UASB-PVA, UASB, and UASB-GAC stayed in the range of 69% to 75% (deviation 1.8%), 46% to 69% (deviation 8.6%), and 61% to 73% (deviation 4.0%), respectively. The highest OLRs reached for the UASB-PVA, UASB, and UASB-GAC were 12, 7, and 8 kg COD/(m3·d), respectively. The performance of the UASB-PVA was the best of the three, the UASB-GAC was second, and the UASB was the worst. High-throughput pyrosequencing analysis showed that Levilinea, Syntrophorhabdus, Desulfovibrio and Acetobacterium were the dominant bacteria in the UASB-PVA, UASB, and UASB-GAC reactors’ granular sludge. The abundance and diversity of the microbial community in the UASB-PVA sludge were higher than for the UASB and UASB-GAC granular sludge.

Keywords

• GAC
• high-throughput sequencing
• pharmaceutical wastewater PVA-gel bead
• UASB reactor