Characterization and Localization of <i>Calb</i>2 in Both the Testis and Ovary of the Japanese Flounder (<i>Paralichthys olivaceus</i>)
Yuting Xiang,
Yahui Wu,
Haoran Zhang,
Jikui Wu,
Junling Zhang
Affiliations
Yuting Xiang
Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education; Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding; Shanghai Ocean University, Shanghai 201306, China
Yahui Wu
Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education; Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding; Shanghai Ocean University, Shanghai 201306, China
Haoran Zhang
Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education; Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding; Shanghai Ocean University, Shanghai 201306, China
Jikui Wu
Laboratory of Quality and Safety Risk Assessment for Aquatic Product on Storage and Preservation, Ministry of Agriculture; National R&D Branch Center for Freshwater Aquatic Products Processing Technology (Shanghai); Shanghai Ocean University, Shanghai 201306, China
Junling Zhang
Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education; Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding; Shanghai Ocean University, Shanghai 201306, China
Although its function in mammalian gonads has been gradually recognized, the expression and function of calretinin (CALB2)—a Ca2+-binding protein—in the testis and ovary of fish are still unclear. Here, we identified the cDNA sequences of calb2 in Paralichthys olivaceus (P. olivaceus); analyzed its gene structure and phylogenetic and syntenic relationship by bioinformatics; and investigated its tissue distribution and localization in the gonads by real-time PCR, western blotting, and immunohistochemistry. The P. olivaceuscalb2 gene has 11 exons and 10 introns, and the full-length cDNA is 1457 bp, including an open reading frame (ORF) of 816 bp encoding 271 amino acids. The CALB2 of P. olivaceus has a higher homology with Lates calcarifer (99%) compared with other species. The conserved synteny of calb2 neighboring gene loci was also detected in fish. Real-time PCR showed that the expression of calb2 mRNA is abundant not only in the brain, but also in the gonads, and exhibits a higher expression in the testis than in the ovary. Western blotting indicated that the CALB2 protein has a higher expression in the testis compared with the ovary. Immunohistochemistry demonstrated that the CALB2 protein appears in Leydig cells and the ovarian germ epithelium. These results reveal that calb2 plays an important role in the gonads of P. olivaceus.
