PDE4 and Epac1 Synergistically Promote Rectal Carcinoma via the cAMP Pathway

Xiangyu Kong; Ganghao Ai; Dai Wang; Renzhen Chen; Dongbei Guo; Youliang Yao; Kai Wang; Guiye Liang; Fengjie Qi; Wenzhi Liu; Yongxing Zhang

doi:10.1155/2019/7145198

Analytical Cellular Pathology (Jan 2019)

PDE4 and Epac1 Synergistically Promote Rectal Carcinoma via the cAMP Pathway

Xiangyu Kong,
Ganghao Ai,
Dai Wang,
Renzhen Chen,
Dongbei Guo,
Youliang Yao,
Kai Wang,
Guiye Liang,
Fengjie Qi,
Wenzhi Liu,
Yongxing Zhang

Affiliations

Xiangyu Kong: Department of Gastrointestinal Surgery, Affiliated Zhongshan Hospital of Dalian University, Dalian, 116001 Liaoning, China
Ganghao Ai: Department of Gastrointestinal Surgery, Affiliated Zhongshan Hospital of Dalian University, Dalian, 116001 Liaoning, China
Dai Wang: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, 361102 Fujian, China
Renzhen Chen: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, 361102 Fujian, China
Dongbei Guo: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, 361102 Fujian, China
Youliang Yao: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, 361102 Fujian, China
Kai Wang: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, 361102 Fujian, China
Guiye Liang: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, 361102 Fujian, China
Fengjie Qi: Department of Pathology, The third Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518001, China
Wenzhi Liu: Department of Gastrointestinal Surgery, Affiliated Zhongshan Hospital of Dalian University, Dalian, 116001 Liaoning, China
Yongxing Zhang: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, 361102 Fujian, China

DOI: https://doi.org/10.1155/2019/7145198
Journal volume & issue: Vol. 2019

Abstract

Read online

Objective. To assess the expression levels of exchange protein 1 directly activated by cAMP (Epac1) and phosphodiesterase 4 (PDE4) in rectal carcinoma, and their associations with clinicopathological indexes. In addition, the associations of PDE4 and Epac1 with A-kinase anchor protein 95, connexin 43, cyclin D1, and cyclin E1 were evaluated. Methods. The PV-9000 two-step immunohistochemistry method was used to determine protein expression in 44 rectal carcinoma tissue samples and 16 paracarcinoma tissue specimens. Results. The positive rate of PDE4 protein expression in rectal carcinoma tissues was higher than that of paracarcinoma tissues (59.09% vs. 12.5%, P0.05). Correlations between PDE4 and Epac1, PDE4 and Cx43, PDE4 and cyclin E1, and Epac1 and Cx43 were observed (all P0.05). Conclusion. PDE4 and Epac1 expression levels are increased in rectal carcinoma tissues, suggesting that the two proteins may be involved in the development of this malignancy. Meanwhile, correlations between PDE4 and Epac1, PDE4 and Cx43, PDE4 and cyclin E1, and Epac1 and Cx43 suggested synergistic effects of these proteins in promoting rectal carcinoma.

Published in Analytical Cellular Pathology

ISSN: 2210-7177 (Print); 2210-7185 (Online)
Publisher: Hindawi Limited
Country of publisher: United Kingdom
LCC subjects: Medicine: Internal medicine: Neoplasms. Tumors. Oncology. Including cancer and carcinogens; Science: Biology (General): Cytology
Website: https://onlinelibrary.wiley.com/journal/2641

About the journal