Anti-Fibronectin Aptamer Modifies Blood Clot Pattern and Stimulates Osteogenesis: An Ex Vivo Study
Natacha Malu Miranda da Costa,
Ludovica Parisi,
Benedetta Ghezzi,
Lisa Elviri,
Sergio Luis Scombatti de Souza,
Arthur Belém Novaes,
Paulo Tambasco de Oliveira,
Guido Maria Macaluso,
Daniela Bazan Palioto
Affiliations
Natacha Malu Miranda da Costa
Department of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirão Preto, University of São Paulo, Avenida Do Café-Subsetor Oeste-11 (N-11), Ribeirão Preto 14040-904, SP, Brazil
Ludovica Parisi
Laboratory for Oral Molecular Biology, Department of Orthodontics and Dentofacial Orthopedics, University of Bern, Freiburgstrasse 3, 3010 Bern, Switzerland
Benedetta Ghezzi
Centro Universitario di Odontoiatria, Dipartimento di Medicina e Chirurgia, University of Parma, Via Gramsci 14, 43126 Parma, Italy
Lisa Elviri
Istituto dei Materiali per l’Elettronica ed il Magnetismo, Consiglio Nazionale Delle Ricerche, Parco Area Delle Scienze 37/A, 43124 Parma, Italy
Sergio Luis Scombatti de Souza
Department of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirão Preto, University of São Paulo, Avenida Do Café-Subsetor Oeste-11 (N-11), Ribeirão Preto 14040-904, SP, Brazil
Arthur Belém Novaes
Department of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirão Preto, University of São Paulo, Avenida Do Café-Subsetor Oeste-11 (N-11), Ribeirão Preto 14040-904, SP, Brazil
Paulo Tambasco de Oliveira
Department of Basic and Oral Biology, School of Dentistry of Ribeirão Preto, University of São Paulo, Avenida Do Café-Subsetor Oeste-11 (N-11), Ribeirão Preto 14040-904, SP, Brazil
Guido Maria Macaluso
Dipartimento di Scienze Degli Alimenti e del Farmaco, Parco Area Delle Scienze 27/A, 43124 Parma, Italy
Daniela Bazan Palioto
Department of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirão Preto, University of São Paulo, Avenida Do Café-Subsetor Oeste-11 (N-11), Ribeirão Preto 14040-904, SP, Brazil
Background: Scaffold (SCA) functionalization with aptamers (APT) provides adsorption of specific bioactive molecules on biomaterial surfaces. The aim of this study was to observe if SCA enriched with anti-fibronectin APT can favor coagulum (PhC) and osteoblasts (OSB) differentiation. Methods: 20 μg of APT was functionalized on SCA by simple adsorption. For PhC formation, SCAs were inserted into rat calvaria defects for 17 h. Following proper transportation (buffer solution PB), OSBs (UMR-106 lineage) were seeded over PhC + SCAs with and without APT. Cells and PhC morphology, PhC cell population, protein labeling and gene expression were observed in different time points. Results: The APT induced higher alkaline phosphatase and bone sialoprotein immunolabeling in OSB. Mesenchymal stem cells, leukocytes and lymphocytes cells were detected more in the APT group than when scaffolds were not functionalized. Additionally, an enriched and dense fibrin network and different cell types were observed, with more OSB and white blood cells in PhC formed on SCA with APT. The gene expression showed higher transforming growth factor beta 1 (TGF-b1) detection in SCA with APT. Conclusions: The SCA functionalization with fibronectin aptamers may alter key morphological and functional features of blood clot formation, and provides a selective expression of proteins related to osteo differentiation. Additionally, aptamers increase TGF-b1 gene expression, which is highly associated with improvements in regenerative therapies.
