Integrated System Based on Thin Film Technologies for Cell-Based Bioluminescence Assays

Domenico Caputo; Giulia Petrucci; Valeria Di Fiore; Alessio Buzzin; Marco Nardecchia; Luca Cevenini; Elisa Michelini; Mara Mirasoli; Aldo Roda; Nicola Lovecchio; Francesca Costantini; Augusto Nascetti; Giampiero de Cesare

doi:10.3390/proceedings1040513

Proceedings (Aug 2017)

Integrated System Based on Thin Film Technologies for Cell-Based Bioluminescence Assays

Domenico Caputo,
Giulia Petrucci,
Valeria Di Fiore,
Alessio Buzzin,
Marco Nardecchia,
Luca Cevenini,
Elisa Michelini,
Mara Mirasoli,
Aldo Roda,
Nicola Lovecchio,
Francesca Costantini,
Augusto Nascetti,
Giampiero de Cesare

Affiliations

Domenico Caputo: Department of Information Engineering, Electronics and Telecommunications, Sapienza University of Rome, Rome, Italy
Giulia Petrucci: Department of Information Engineering, Electronics and Telecommunications, Sapienza University of Rome, Rome, Italy
Valeria Di Fiore: Department of Information Engineering, Electronics and Telecommunications, Sapienza University of Rome, Rome, Italy
Alessio Buzzin: Department of Information Engineering, Electronics and Telecommunications, Sapienza University of Rome, Rome, Italy
Marco Nardecchia: Department of Information Engineering, Electronics and Telecommunications, Sapienza University of Rome, Rome, Italy
Luca Cevenini: Department of Chemistry “G. Ciamician”, Alma Mater Studiorum—University of Bologna, Bologna, Italy
Elisa Michelini: Department of Chemistry “G. Ciamician”, Alma Mater Studiorum—University of Bologna, Bologna, Italy
Mara Mirasoli: Department of Chemistry “G. Ciamician”, Alma Mater Studiorum—University of Bologna, Bologna, Italy
Aldo Roda: Department of Chemistry “G. Ciamician”, Alma Mater Studiorum—University of Bologna, Bologna, Italy
Nicola Lovecchio: Department of Information Engineering, Electronics and Telecommunications, Sapienza University of Rome, Rome, Italy
Francesca Costantini: Department of Information Engineering, Electronics and Telecommunications, Sapienza University of Rome, Rome, Italy
Augusto Nascetti: School of Aerospace Engineering, Sapienza University of Rome, Rome, Italy
Giampiero de Cesare: Department of Information Engineering, Electronics and Telecommunications, Sapienza University of Rome, Rome, Italy

DOI: https://doi.org/10.3390/proceedings1040513
Journal volume & issue: Vol. 1, no. 4
p. 513

Abstract

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This work presents a miniaturized lab-on-chip system suitable for monitoring the activity of living cells through the on-chip detection of their bioluminescence emission. The system integrates amorphous silicon diodes, acting as temperature and light sensors, and indium tin oxide film, acting as heater, on a single glass substrate. During its operation, the glass is thermally and optically coupled to the investigated cells and electrically connected to an electronic board, which controls the lab-on-chip temperature and monitors the sensor photocurrents. The proposed lab-on-chip is particularly attractive for the development of portable cell-based biosensors useful for biological activity monitoring as well as for cell cytotoxicity evaluation.

Published in Proceedings

ISSN: 2504-3900 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: General Works
Website: http://www.mdpi.com/journal/proceedings

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