International Journal of General Medicine (Dec 2020)

Circular RNA 0001313 Knockdown Suppresses Non-Small Cell Lung Cancer Cell Proliferation and Invasion via the microRNA-452/HMGB3/ERK/MAPK Axis

  • Zhang S,
  • Liu J,
  • Yuan T,
  • Liu H,
  • Wan C,
  • Le Y

Journal volume & issue
Vol. Volume 13
pp. 1495 – 1507

Abstract

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Shihao Zhang,* Jiansheng Liu,* Taiwen Yuan, Huiyu Liu, Chengwei Wan, Yonghong Le Department of Respiratory and Critical Care Medicine, Ganzhou People’s Hospital, Ganzhou 341000, Jiangxi, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yonghong LeDepartment of Respiratory and Critical Care Medicine, Ganzhou People’s Hospital, No. 17, Hongqi Avenue, Zhanggong District, Ganzhou 341000, Jiangxi, People’s Republic of ChinaTel/Fax +8613879769189Email [email protected]: Non-small cell lung cancer (NSCLC) seriously endangers human health. Circular RNAs (circRNAs) regulate diverse types of cancers, including NSCLC. This study investigated the possible mechanism of circ0001313 in NSCLC.Materials and Methods: Circ0001313 expression in NSCLC tissues was measured, and its correlation with clinicopathological features was analyzed. The binding relationships among circ0001313, microRNA (miR)-452 and HMGB3 were tested. The gain and loss of functions were performed to examine NSCLC cell malignant behaviors. After HMGB3 overexpression, ERK/MAPK pathway-related protein levels were detected. Subsequently, the rescue experiment was further performed using an ERK/MAPK pathway inhibitor PD98059.Results: Abnormally elevated circ0001313 and decreased miR-452 in NSCLC cells were observed. Circ0001313 silencing or miR-452 overexpression significantly reduced NSCLC cell proliferation and invasion. Circ0001313 competitively bound to miR-452 to upregulate HMGB3, thus promoting NSCLC cell growth. HMGB3 overexpression activated the ERK/MAPK pathway to contribute to NSCLC development.Conclusion: We highlighted that silencing of circ0001313 blunted the ERK/MAPK pathway via the miR-452/HMGB3 axis, thereby inhibiting NSCLC cell proliferation and invasion.Keywords: non-small cell lung cancer, circular RNA 0001313, microRNA-452, HMGB3, ERK/MAPK pathway, proliferation and invasion

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