Genetic stability analysis and molecular depiction in elite entries of rice (Oryza sativa L.)

Abstract

Abstract Background Genetic stability is considered one of the most important genetic tests used to ascertain the extent of genetic stability reached in plants; consequently, the goal of this research is to detect the degree of genetic stability of a group of a superior rice lines under different climatic environments. Results The seven entries of rice exhibited highly genetic stability depending on the results obtained from genetic stability analysis where they were recorded as high yielding; in addition, positive data for the remaining traits studied under the 12 environmental conditions were tested. Line numbers 1, 3, 4, and 5 were in the first rank for high genetic stability and high stable yielding under all experiments, while line numbers 2, 6, and 7 were recorded in the second rank. The values of broad sense heritability were very high in some traits (plant height, heading date, number of filled grains/panicle, grain yield/plant, and flag leaf area) which indicated that the genetic variance played an important role for controlling and inheriting these traits. A total of 101 fragments were generated using six primers of ISSR through comparison among the seven rice lines, where 34 of them were monomorphic and 67 bands were polymorphic with 66.33% polymorphism. Conclusion From the previous results, it could be concluded that the seven promising lines showed high genetic stability and recorded highly stable yielding under various environments which confirmed their importance in rice breeding programs for enhancing salinity tolerance, resistance to many diseases, and other stresses under Egyptian conditions.

Keywords

• Rice
• stable yield
• stability analysis
• broad sense heritability
• genotypic variation
• phenotypic variation