Effects of Substrate and Exogenous Auxin on the Adventitious Rooting of Dianthus caryophyllus L.

Abstract

Abstract In this study, Dianthus caryophyllus L. was used as the experimental plant to investigate the effects of rooting substrate and exogenous auxin concentration on the adventitious rooting of the stem cuttings. Our results showed that the formulated substrates with different physicochemical properties significantly affected the root formation. The substrate with a ratio of cocopeat to perlite at 1:1 (v:v) resulted in the optimum rooting of D. caryophyllus cuttings. Different Indole-3-butyric acid (IBA) and 1-naphthalene acetic acid (NAA) concentrations affected the rooting percentage and seedling rate of D. caryophyllus. Application of NAA at 1000 mg·kg−1 with IBA at 100 mg·kg−1 resulted in the greatest rooting percentage and improved breeding speed. The rooting percentage and seedling rate did not increase with the increase in auxin concentration. Based on these results, we concluded that an appropriate rooting substrate is required to fulfill proper rooting of D. caryohhyllus cuttings, whereas an exogenous application of IBA and NAA at 1000 mg·kg−1 and 100 mg·kg−1 promoted the rooting and a higher auxin concertation inhibited rooting.

Keywords

• auxin concentration; substrate formulation