STAR Protocols (Mar 2021)

Protocol for generation of lung adenocarcinoma organoids from clinical samples

  • Zhichao Li,
  • Lei Yu,
  • Dong Chen,
  • Zhaoyue Meng,
  • Wei Chen,
  • Weiren Huang

Journal volume & issue
Vol. 2, no. 1
p. 100239

Abstract

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Summary: Human cancer tissue-derived organoids maintain the mutational spectrum and histological characteristics of their parental tumors and thus provide a platform for predicting patients’ responses to anticancer drugs. Here, we provide a fully detailed, step-by-step protocol to derive lung adenocarcinoma organoids from primary tumor tissues. Organoid lines can be generated with a success rate of 80% using our protocol.For complete details on the use and execution of this protocol, please refer to Li et al. (2020).

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