Protocol for generation of lung adenocarcinoma organoids from clinical samples

Zhichao Li; Lei Yu; Dong Chen; Zhaoyue Meng; Wei Chen; Weiren Huang

STAR Protocols (Mar 2021)

Protocol for generation of lung adenocarcinoma organoids from clinical samples

Zhichao Li,
Lei Yu,
Dong Chen,
Zhaoyue Meng,
Wei Chen,
Weiren Huang

Affiliations

Zhichao Li: Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518035, China; International Cancer Center, Shenzhen University School of Medicine, Shenzhen, Guangdong 518060, China; Guangdong Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors, Shenzhen, Guangdong 518035, China
Lei Yu: Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518035, China; International Cancer Center, Shenzhen University School of Medicine, Shenzhen, Guangdong 518060, China; Guangdong Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors, Shenzhen, Guangdong 518035, China
Dong Chen: Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518035, China
Zhaoyue Meng: Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518035, China
Wei Chen: Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518035, China; International Cancer Center, Shenzhen University School of Medicine, Shenzhen, Guangdong 518060, China; Guangdong Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors, Shenzhen, Guangdong 518035, China; The First Affiliated Hospital of Shantou University, Shantou, Guangdong 515041, China; Corresponding author
Weiren Huang: Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518035, China; International Cancer Center, Shenzhen University School of Medicine, Shenzhen, Guangdong 518060, China; Guangdong Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors, Shenzhen, Guangdong 518035, China; Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong 518055, China; The First Affiliated Hospital of Shantou University, Shantou, Guangdong 515041, China; Corresponding author

Journal volume & issue: Vol. 2, no. 1
p. 100239

Abstract

Read online

Summary: Human cancer tissue-derived organoids maintain the mutational spectrum and histological characteristics of their parental tumors and thus provide a platform for predicting patients’ responses to anticancer drugs. Here, we provide a fully detailed, step-by-step protocol to derive lung adenocarcinoma organoids from primary tumor tissues. Organoid lines can be generated with a success rate of 80% using our protocol.For complete details on the use and execution of this protocol, please refer to Li et al. (2020).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

About the journal

Abstract

Keywords