Comparison of the Effect of Fertilization with Ash from Wood Chips on Bacterial Community in Podzolic and Chernozem Soils for the Cultivation of Winter Oilseed Rape: A Preliminary Study
Jadwiga Stanek-Tarkowska,
Miłosz Pastuszczak,
Ewa Szpunar-Krok,
Miroslava Kačániová,
Maciej Ireneusz Kluz,
Ewa Antonina Czyż,
Rafał Pieniążek,
Karol Skrobacz,
Karol Pietrzyk
Affiliations
Jadwiga Stanek-Tarkowska
Department of Soil Science, Environmental Chemistry and Hydrology, University of Rzeszow, Zelwerowicza 8B, 35-601 Rzeszów, Poland
Miłosz Pastuszczak
Department of Soil Science, Environmental Chemistry and Hydrology, University of Rzeszow, Zelwerowicza 8B, 35-601 Rzeszów, Poland
Ewa Szpunar-Krok
Department of Plant Production, College of Natural Sciences, University of Rzeszów, Zelwerowicza 4, 35-601 Rzeszów, Poland
Miroslava Kačániová
Department of Bioenergetics, Food Analysis and Microbiology, Institute of Food and Nutrition Technology, University of Rzeszow, 35-959 Rzeszow, Poland
Maciej Ireneusz Kluz
Department of Bioenergetics, Food Analysis and Microbiology, Institute of Food and Nutrition Technology, University of Rzeszow, 35-959 Rzeszow, Poland
Ewa Antonina Czyż
Department of Soil Science, Environmental Chemistry and Hydrology, University of Rzeszow, Zelwerowicza 8B, 35-601 Rzeszów, Poland
Rafał Pieniążek
Department of Soil Science, Environmental Chemistry and Hydrology, University of Rzeszow, Zelwerowicza 8B, 35-601 Rzeszów, Poland
Karol Skrobacz
Department of Soil Science, Environmental Chemistry and Hydrology, University of Rzeszow, Zelwerowicza 8B, 35-601 Rzeszów, Poland
Karol Pietrzyk
Department of Bioenergetics, Food Analysis and Microbiology, Institute of Food and Nutrition Technology, University of Rzeszow, 35-959 Rzeszow, Poland
The aim of the research was to investigate whether different doses of ash from biomass combustion (Salix viminalis L. willow) have an impact on the number and community of soil bacteria. The experiment was carried out on podzolic and chernozem soils in a one-way field experiment (control, NPK, 100, 200, 300, 400, 500 kg K2Oha−1). The ash from the biomass was characterized by pH 12.83 ± 0.68 and high content of macronutrients. Samples were taken from the 0–5 cm layer of soil under the cultivation of winter oilseed rape (Brassica napus L. var. Napus) in April and September 2021. The plate count method with PCA solid medium was used to determine the number of microorganisms, and mass spectrometry (MALDI-TOF MS) was used to analyze the microbiological community. The research showed an increase in the number of microorganisms after the use of the biomass ash fertilizer in the variants with ash doses from 200 to 500 kg K2Oha−1. The highest amount of soil bacteria in both tested soils was determined in these variants. In total, 44 bacterial species of 5 genera were identified in all variants: Bacillus, Paenarthrobacter, Pseudarthrobacter, Pseudomonas, and Rhodococcus. An important factor in the growth of the number of bacteria, in addition to the dose of biomass ash, was soil moisture, which in September was significantly higher than in April 2021 in both soils.
