Expression Of CK19 Gene In Patients With Lung Cancer And Its Comparison With Carcinoembryonic Antigen In Peripheral Blood
Abdolreza Mohamadnia,
Shirin Karimi,
Reza Yadegar Azari,
Seyed Alireza Naji,
Adnan Khosravi,
Naghmeh Bahrami,
Massoud Saidijam
Affiliations
Abdolreza Mohamadnia
Assistant Professor, Virology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Biotechnology & Molecular Medicine Department, School of Advanced Technologies, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Shirin Karimi
Associate Professor, Mycobacteriology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Dr Masih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Reza Yadegar Azari
Assistant Professor, Genetics and Molecular Medicine Department, School of Medicine, Molecular Medicine Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
Seyed Alireza Naji
Associate Professor ,Virology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Dr Masih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Adnan Khosravi
Assistant Professor, Tobacco Prevention and Control Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Dr Masih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Naghmeh Bahrami
Assistant Professor, Cranioniomaxillofacial Research Center, Oral and Maxillofacial Surgery Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
Massoud Saidijam
Professor, Molecular Medicine and Genetics Department, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Background and Aim: Lung cancer is the most common cancer among men around the world. The aim of this study was to determine the expression levels of CK19 mRNA marker in the affected patients’ peripheral blood. Materials and Methods: Thirty patients with lung cancer (NSCLC type) were compared with 30 healthy controls. After taking peripheral blood samples and extracting total RNA, cDNA was synthesized and examined by Real-time RT-PCR technique. Then, the CEA antigen was measured by ELISA. Results: The CK19mRNA was positive in 7 out of 30 lung cancer patients. Hence, its sensitivity was determined to be 23.3 %. The serum level of CEA antigen was positive in 11 out of 30 lung cancer patients. The mean serum level of CEA antigen markers was higher in patients than in controls; the difference was statistically significant (P< 0.001). The sensitivity of this test was determined to be 36.6 %. Conclusion: This study showed that the sensitivity of CK19 mRNA in peripheral blood was low, but it had a high specificity for the diagnosis of primary lung cancer. Also, it was found that CEA antigen could be the specific marker for the early detection of lung cancer in patients’ peripheral blood.
