THE PROPERTIES OF THE CONIDIA OF STRAINS OF THE ACTINOMYCETE <i>STREPTOMYCES LUCENSIS</i> AND <i>STREPTOMYCES VIOLACEUS</i> DURING STORAGE AT LOW TEMPERATURES

Abstract

Collection cultures of actinomycetes are mainly stored in a dried state on adsorbents. Practiced low-temperature storage of actinomycetes at minus 70°C. The article presents the results of investigations of the properties of the conidia of strains of the actinomycete Streptomyces lucensis VKPM Ac–1743 and Streptomyces violaceus VKPM Ac–1734 is in the process of storage at minus 12°C and minus 18°C in glycerin solution and in 0,9 % aqueous sodium chloride solution. It was found that the inhibitory activity in the native solution for the studied strains stored in the glycerin solution at minus 12°C and plus 4°C, as a result of their subsequent cultivation on the starch hydrolyzate for 120 h is at the level of (450 ± 10) IE/cm3. The indicator for crops stored at minus 18°C was higher (560 ± 10) IE/cm3. Low-temperature storage of conidia in saline solution is less effective. Pigmentation is more active in the cultivation of strains stored at minus 18°C.

Keywords

• strains of streptomyces lucensis vkpm ас–1743 and streptomyces violaceus vkpm ас–1734
• low-temperature storage
• viability
• inhibitory activity
• pigment formation