E3S Web of Conferences (Jan 2023)
Isolation of cellulolytic bacteria from cultivated soil samples
Abstract
Cellulose decomposition is widespread in all types of soils in the world, which plays an important role in the carbon cycle and the decomposition of plant residues, which is especially important for agriculture. Cellulolytic bacteria can be actively used in the processes of increasing soil fertility, especially in those areas where the natural content of such microorganisms is not high. Within the framework of this work, cellulolytic bacteria were isolated from soil actively used for growing crops. 39 strains of microorganisms were selected, their lytic activity in relation to cellulose compounds was determined. It was observed that 5 strains showed high cellulose activity already within 24 hours. The study of some biological properties of isolated microorganisms showed their strong difference from each other. The unifying feature of all isolated strains was the absence of tryptophan fermentation. The obtained data allow us to continue creating a collection of cellulolytic microorganisms with their further identification.