Memorias do Instituto Oswaldo Cruz (Jul 2020)

Preliminary results of SARS-CoV-2 detection in sewerage system in Niterói municipality, Rio de Janeiro, Brazil

  • Tatiana Prado,
  • Tulio Machado Fumian,
  • Camille Ferreira Mannarino,
  • Adriana Gonçalves Maranhão,
  • Marilda Mendonça Siqueira,
  • Marize Pereira Miagostovich

DOI
https://doi.org/10.1590/0074-02760200196
Journal volume & issue
Vol. 115

Abstract

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This study presents preliminary results from a sewage-based surveillance to monitor the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the municipality of Niterói, State of Rio de Janeiro, Brazil. By using ultracentrifugation method associated to quantitative reverse transcription polymerase chain reaction (RT-qPCR) we detected SARS-CoV-2 in 41.6% (5/12) of raw sewage samples obtained from sewage treatment plants and sewers network in the city. This pioneer study carried out in Brazil aims to subsidise information for health surveillance concerning the viral circulation in different areas of the city and, revealed the insertion and importance of environmental virology in health public policies.

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