Genome-scale chemogenomic CRISPR screens in human cells using the TKOv3 library

Abstract

Summary: CRISPR-based genetic screens revolutionized our ability to genetically probe cell biology. We present a protocol to conduct genome-scale chemogenomic dropout CRISPR screens in the human RPE1-hTERT p53−/− cell line. We use the TKOv3 library, which contains 70,948 sgRNAs targeting 18,053 genes. Here, we describe how to set up the screen, the reagents required, and how to sequence and analyze the results. This protocol can be customized for other libraries, cell lines, and sequencing instruments.For complete details on the use and execution of this protocol, please refer to Olivieri et al. (2020).

Keywords

• CRISPR
• Genetics
• Genomics
• High-throughput screening
• Sequence analysis
• Sequencing