Effective and biocompatible antibacterial surfaces via facile synthesis and surface modification of peptide polymers

Ziyi Lu; Yueming Wu; Zihao Cong; Yuxin Qian; Xue Wu; Ning Shao; Zhongqian Qiao; Haodong Zhang; Yunrui She; Kang Chen; Hengxue Xiang; Bin Sun; Qian Yu; Yuan Yuan; Haodong Lin; Meifang Zhu; Runhui Liu

Bioactive Materials (Dec 2021)

Effective and biocompatible antibacterial surfaces via facile synthesis and surface modification of peptide polymers

Ziyi Lu,
Yueming Wu,
Zihao Cong,
Yuxin Qian,
Xue Wu,
Ning Shao,
Zhongqian Qiao,
Haodong Zhang,
Yunrui She,
Kang Chen,
Hengxue Xiang,
Bin Sun,
Qian Yu,
Yuan Yuan,
Haodong Lin,
Meifang Zhu,
Runhui Liu

Affiliations

Ziyi Lu: State Key Laboratory of Bioreactor Engineering, College of Chemistry, Chemical Engineering and Materials Science, East China University of Science and Technology, Shanghai, 200237, China
Yueming Wu: State Key Laboratory of Bioreactor Engineering, College of Chemistry, Chemical Engineering and Materials Science, East China University of Science and Technology, Shanghai, 200237, China
Zihao Cong: State Key Laboratory of Bioreactor Engineering, College of Chemistry, Chemical Engineering and Materials Science, East China University of Science and Technology, Shanghai, 200237, China
Yuxin Qian: State Key Laboratory of Bioreactor Engineering, College of Chemistry, Chemical Engineering and Materials Science, East China University of Science and Technology, Shanghai, 200237, China
Xue Wu: State Key Laboratory of Bioreactor Engineering, College of Chemistry, Chemical Engineering and Materials Science, East China University of Science and Technology, Shanghai, 200237, China
Ning Shao: State Key Laboratory of Bioreactor Engineering, College of Chemistry, Chemical Engineering and Materials Science, East China University of Science and Technology, Shanghai, 200237, China
Zhongqian Qiao: State Key Laboratory of Bioreactor Engineering, College of Chemistry, Chemical Engineering and Materials Science, East China University of Science and Technology, Shanghai, 200237, China
Haodong Zhang: State Key Laboratory of Bioreactor Engineering, College of Chemistry, Chemical Engineering and Materials Science, East China University of Science and Technology, Shanghai, 200237, China
Yunrui She: State Key Laboratory of Bioreactor Engineering, College of Chemistry, Chemical Engineering and Materials Science, East China University of Science and Technology, Shanghai, 200237, China
Kang Chen: State Key Laboratory of Bioreactor Engineering, College of Chemistry, Chemical Engineering and Materials Science, East China University of Science and Technology, Shanghai, 200237, China
Hengxue Xiang: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, 201620, China
Bin Sun: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, 201620, China
Qian Yu: Jiangsu Key Laboratory of Advanced Functional Polymers Design and Application, Soochow University, Suzhou, 215123, China
Yuan Yuan: Key Laboratory for Ultrafine Materials of Ministry of Education, Frontiers Science Center for Materiobiology and Dynamic Chemistry, Research Center for Biomedical Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, 200237, China
Haodong Lin: Department of Orthopedic Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, China
Meifang Zhu: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, 201620, China
Runhui Liu: State Key Laboratory of Bioreactor Engineering, College of Chemistry, Chemical Engineering and Materials Science, East China University of Science and Technology, Shanghai, 200237, China; Key Laboratory for Ultrafine Materials of Ministry of Education, Frontiers Science Center for Materiobiology and Dynamic Chemistry, Research Center for Biomedical Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, 200237, China; Corresponding author. State Key Laboratory of Bioreactor Engineering, College of Chemistry, Chemical Engineering and Materials Science, East China University of Science and Technology, Shanghai, 200237, China.

Journal volume & issue: Vol. 6, no. 12
pp. 4531 – 4541

Abstract

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It is an urgent need to tackle drug-resistance microbial infections that are associated with implantable biomedical devices. Host defense peptide-mimicking polymers have been actively explored in recent years to fight against drug-resistant microbes. Our recent report on lithium hexamethyldisilazide-initiated superfast polymerization on amino acid N-carboxyanhydrides enables the quick synthesis of host defense peptide-mimicking peptide polymers. Here we reported a facile and cost-effective thermoplastic polyurethane (TPU) surface modification of peptide polymer (DLL: BLG = 90 : 10) using plasma surface activation and substitution reaction between thiol and bromide groups. The peptide polymer-modified TPU surfaces exhibited board-spectrum antibacterial property as well as effective contact-killing ability in vitro. Furthermore, the peptide polymer-modified TPU surfaces showed excellent biocompatibility, displaying no hemolysis and cytotoxicity. In vivo study using methicillin-resistant Staphylococcus aureus (MRSA) for subcutaneous implantation infectious model showed that peptide polymer-modified TPU surfaces revealed obvious suppression of infection and great histocompatibility, compared to bare TPU surfaces. We further explored the antimicrobial mechanism of the peptide polymer-modified TPU surfaces, which revealed a surface contact-killing mechanism by disrupting the bacterial membrane. These results demonstrated great potential of the peptide-modified TPU surfaces for practical application to combat bacterial infections that are associated with implantable materials and devices.

Published in Bioactive Materials

ISSN: 2452-199X (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials; Science: Biology (General)
Website: https://www.keaipublishing.com/en/journals/bioactive-materials/

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