OsPPG encodes a pseudouridine-5′-phosphate glycosidase and regulates rice spikelet development
Rui Chen,
Dagang Tian,
Yarong Lin,
Jiaqing Huang,
Qinqin Yang,
Yupei Li,
Huaqing Liu,
Shaohua Yang,
Jingyang Chen,
Shufen Zhou,
Feng Wang,
Shubiao Zhang
Affiliations
Rui Chen
Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education/Key Laboratory of Biological Breeding for Fujian and Taiwan Crops, Ministry of Agriculture and Rural Affairs, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China; Fujian Key Laboratory of Genetic Engineering for Agriculture, Biotechnology Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, Fujian, China
Dagang Tian
Fujian Key Laboratory of Genetic Engineering for Agriculture, Biotechnology Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, Fujian, China
Yarong Lin
Fujian Key Laboratory of Genetic Engineering for Agriculture, Biotechnology Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, Fujian, China
Jiaqing Huang
Institute of Resources, Environment and Soil Fertilizer, Fujian Academy of Agricultural Sciences, Fuzhou 350013, Fujian, China
Qinqin Yang
Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education/Key Laboratory of Biological Breeding for Fujian and Taiwan Crops, Ministry of Agriculture and Rural Affairs, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
Yupei Li
Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education/Key Laboratory of Biological Breeding for Fujian and Taiwan Crops, Ministry of Agriculture and Rural Affairs, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China; Fujian Key Laboratory of Genetic Engineering for Agriculture, Biotechnology Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, Fujian, China
Huaqing Liu
Fujian Key Laboratory of Genetic Engineering for Agriculture, Biotechnology Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, Fujian, China
Shaohua Yang
Fujian Key Laboratory of Genetic Engineering for Agriculture, Biotechnology Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, Fujian, China
Jingyang Chen
Fujian Key Laboratory of Genetic Engineering for Agriculture, Biotechnology Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, Fujian, China
Shufen Zhou
Fujian Key Laboratory of Genetic Engineering for Agriculture, Biotechnology Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, Fujian, China
Feng Wang
Fujian Key Laboratory of Genetic Engineering for Agriculture, Biotechnology Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, Fujian, China; Corresponding authors.
Shubiao Zhang
Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education/Key Laboratory of Biological Breeding for Fujian and Taiwan Crops, Ministry of Agriculture and Rural Affairs, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China; Corresponding authors.
Florets are the basic structural units of spikelets, and their morphogenesis determines the yield and quality of rice grains. However, whether and how pseudouridine-5′-phosphate glycosidase participates in rice spikelet development remains an open question. In this study, we identified a novel gene, OsPPG, which encodes a peroxisome-localized pseudouridine-5′-phosphate glycosidase and regulates the development of rice spikelets. osppg mutants exhibited abnormal sterile lemma, lemma, palea, lodicule, stamens, and pistils; male sterility; shorter panicles; and reduced plant height. OsPPG was found to regulate several OsMADS genes, thereby affecting the morphogenesis of rice spikelets. Furthermore, metabolomics revealed that the OsPPG gene was involved in the decomposition of pseudouridine via the pyrimidine metabolism pathway and may affect the jasmonic acid signaling pathway. These results suggest that OsPPG is a key regulator of rice spikelet development.
