Protective Effect of Cannabinoid Type 2 Receptor Agonist Against Ovarian Ischemia/reperfusion Injury in Rats

Abstract

Objective: The aim of this study is to prove whether or not the cannabinoid (CB)2 receptor agonist, which is known to be effective in anti-inflammatory effects and tried in various organ and/or tissue ischemia/reperfusion (I/R) injuries, works in the treatment of I/R injury, which is a replication of the damage caused by clinical ovarian torsion.Materials and Methods: Rats were divided into five groups (n=6). After anesthesia, an approximately 2-cm incision was made in the lower abdominal region of the rats and both ovaries, uterine horns, and adnexa were occluded with a clamp for 30 min, except for the sham control group. After 3 h, both ovaries were surgically removed. Blood was collected for the measurement of cytokine levels in the serum.Results: The induction of ovaries by I/R injury resulted in an increase in interleukin 1 beta, tumor necrosis factor alpha, and malondialdehyde levels and decrease in glutathione (GSH) levels in ovarian tissues and serum. The pretreatment of the CB2 receptor agonist decreased these cytokines levels in both ovarian tissues and serum. The GSH level in both was reduced by I/R injury, while the agonist increased the GSH level reduced by I/R injury. The administration of the antagonist reversed the curative effect of the agonist. Conclusion: The results of this study support that the CB2 receptor agonist can be used to treat I/R injury, which is an imitation of ovarian torsion. However, no the clinical trial is a limitation of this study.

Keywords

• i/r
• am1241
• ovarian torsion
• cytokines
• am630
• i/r
• am1241
• ovaryum torsiyonu
• sitokinler
• am630