Frontiers in Cell and Developmental Biology (Sep 2024)
Standardization and quality assessment for human intestinal organoids
- Hana Lee,
- Hana Lee,
- Seunghye Yang,
- Seunghye Yang,
- Kyung Jin Lee,
- Kyung Jin Lee,
- Si-Na Kim,
- Ji-Seon Jeong,
- Ji-Seon Jeong,
- Ki Young Kim,
- Ki Young Kim,
- Cho-Rok Jung,
- Cho-Rok Jung,
- Cho-Rok Jung,
- Sooyeon Jeon,
- Dayeon Kwon,
- Sungin Lee,
- Hanbyeol Lee,
- Chihye Park,
- Sun-Ju Ahn,
- Sun-Ju Ahn,
- Jongman Yoo,
- Jongman Yoo,
- Jongman Yoo,
- Mi-Young Son,
- Mi-Young Son,
- Mi-Young Son,
- Mi-Young Son
Affiliations
- Hana Lee
- Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea
- Hana Lee
- Organoid Standards Initiative (OSI), Department of Biophysics, Institute of Quantum Biophysics, Sungkyunkwan University, Suwon, Republic of Korea
- Seunghye Yang
- Organoid Standards Initiative (OSI), Department of Biophysics, Institute of Quantum Biophysics, Sungkyunkwan University, Suwon, Republic of Korea
- Seunghye Yang
- ORGANOIDSCIENCES, Seongnam-si, Republic of Korea
- Kyung Jin Lee
- Organoid Standards Initiative (OSI), Department of Biophysics, Institute of Quantum Biophysics, Sungkyunkwan University, Suwon, Republic of Korea
- Kyung Jin Lee
- ORGANOIDSCIENCES, Seongnam-si, Republic of Korea
- Si-Na Kim
- ORGANOIDSCIENCES, Seongnam-si, Republic of Korea
- Ji-Seon Jeong
- Organoid Standards Initiative (OSI), Department of Biophysics, Institute of Quantum Biophysics, Sungkyunkwan University, Suwon, Republic of Korea
- Ji-Seon Jeong
- Korea Research Institute of Standards and Science (KRISS), Daejeon, Republic of Korea
- Ki Young Kim
- Organoid Standards Initiative (OSI), Department of Biophysics, Institute of Quantum Biophysics, Sungkyunkwan University, Suwon, Republic of Korea
- Ki Young Kim
- Therapeutics and Biotechnology Division, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea
- Cho-Rok Jung
- Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea
- Cho-Rok Jung
- Organoid Standards Initiative (OSI), Department of Biophysics, Institute of Quantum Biophysics, Sungkyunkwan University, Suwon, Republic of Korea
- Cho-Rok Jung
- KRIBB School of Bioscience, Korea University of Science and Technology (UST), Daejeon, Republic of Korea
- Sooyeon Jeon
- Digital Health Laboratory, Department of Biophysics, Sungkyunkwan University, Suwon, Republic of Korea
- Dayeon Kwon
- Digital Health Laboratory, Department of Biophysics, Sungkyunkwan University, Suwon, Republic of Korea
- Sungin Lee
- Digital Health Laboratory, Department of Biophysics, Sungkyunkwan University, Suwon, Republic of Korea
- Hanbyeol Lee
- Digital Health Laboratory, Department of Biophysics, Sungkyunkwan University, Suwon, Republic of Korea
- Chihye Park
- Digital Health Laboratory, Department of Biophysics, Sungkyunkwan University, Suwon, Republic of Korea
- Sun-Ju Ahn
- Organoid Standards Initiative (OSI), Department of Biophysics, Institute of Quantum Biophysics, Sungkyunkwan University, Suwon, Republic of Korea
- Sun-Ju Ahn
- Digital Health Laboratory, Department of Biophysics, Sungkyunkwan University, Suwon, Republic of Korea
- Jongman Yoo
- Organoid Standards Initiative (OSI), Department of Biophysics, Institute of Quantum Biophysics, Sungkyunkwan University, Suwon, Republic of Korea
- Jongman Yoo
- ORGANOIDSCIENCES, Seongnam-si, Republic of Korea
- Jongman Yoo
- Department of Microbiology, CHA University School of Medicine, Seongnam-si, Republic of Korea
- Mi-Young Son
- Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea
- Mi-Young Son
- Organoid Standards Initiative (OSI), Department of Biophysics, Institute of Quantum Biophysics, Sungkyunkwan University, Suwon, Republic of Korea
- Mi-Young Son
- KRIBB School of Bioscience, Korea University of Science and Technology (UST), Daejeon, Republic of Korea
- Mi-Young Son
- Department of Biological Science, Sungkyunkwan University, Suwon, Republic of Korea
- DOI
- https://doi.org/10.3389/fcell.2024.1383893
- Journal volume & issue
-
Vol. 12
Abstract
To enhance the practical application of intestinal organoids, it is imperative to establish standardized guidelines. This proposed standardization outlines a comprehensive framework to ensure consistency and reliability in the development, characterization, and application of intestinal organoids. The recommended guidelines encompass crucial parameters, including culture conditions, critical quality attributes, quality control measures, and functional assessments, aimed at fostering a standardized approach across diverse research initiatives. The implementation of these guidelines is anticipated to significantly contribute to the reproducibility and comparability of results in the burgeoning field of intestinal organoid research.
Keywords
- standardization
- guideline
- human adult stem cell (hASC)
- human pluripotent stem cell (hPSC)
- human intestinal organoid (hIO)
