Preparation of Foam Dressings Based on Gelatin, Hyaluronic Acid, and Carboxymethyl Chitosan Containing Fibroblast Growth Factor-7 for Dermal Regeneration
Longhao Jin,
Sun-Jung Yoon,
Dae Hoon Lee,
Yun Chang Pyun,
Woo Youp Kim,
Ju Hwa Lee,
Gilson Khang,
Heung Jae Chun,
Dae Hyeok Yang
Affiliations
Longhao Jin
Department of Orthopedic Surgery, Yanbian University Hospital, Yanji 133000, China
Sun-Jung Yoon
Department of Orthopedic Surgery, Research Institute of Clinical Medicine of Jeonbuk National University, Biomedical Research Institute of Jeonbuk National University Hospital, Jeonbuk National University Medical School, Jeonju 54896, Korea
Dae Hoon Lee
Department of Bionanotechnology and Bioconvergence Engineering, Department of Polymer Nano Science and Technology, Jeonbuk National University, Jeonju 54896, Korea
Yun Chang Pyun
Department of Bionanotechnology and Bioconvergence Engineering, Department of Polymer Nano Science and Technology, Jeonbuk National University, Jeonju 54896, Korea
Woo Youp Kim
Department of Bionanotechnology and Bioconvergence Engineering, Department of Polymer Nano Science and Technology, Jeonbuk National University, Jeonju 54896, Korea
Ju Hwa Lee
Department of Bionanotechnology and Bioconvergence Engineering, Department of Polymer Nano Science and Technology, Jeonbuk National University, Jeonju 54896, Korea
Gilson Khang
Department of Bionanotechnology and Bioconvergence Engineering, Department of Polymer Nano Science and Technology, Jeonbuk National University, Jeonju 54896, Korea
Heung Jae Chun
Department of Medical Life Sciences, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea
Dae Hyeok Yang
Institute of Cell and Tissue Engineering, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea
Wound recovery close to the function of the native skin is the goal of wound healing. In this study, we prepared foam dressings (FDs; 2-GHC-FD-1–9, 5-GHC-FD-1–9, and 10-GHC-FD-1–9) composed of various concentrations of gelatin, hyaluronic acid, and carboxymethyl chitosan, which are chemically interconnected through amide bond formation, for evaluating wound healing. Tensile and cell proliferation tests showed that 2-GHC-FD-1–9 are suitable for wound dressing. For further evaluation, three types of FDs, 2-GHC-FD-1, 2-GHC-FD-4, and 2-GHC-FD-8 were chosen. The results of animal intradermal reactivity, water vapor transmission rate, and absorption rate of the three FDs indicated that 2-GHC-FD-8 is the most appropriate scaffold for wound healing. For wound healing acceleration, various concentrations of fibroblast growth factor-7 (FGF-7) was soaked in 2-GHC-FD-8 (2-GHC-FD-8/F1-6) and evaluated by using scanning electron microscopy, cell proliferation, release behavior, and in vivo animal tests. The FDs showed interconnected porous structures, increased cell proliferation until 8.0 × 10−11 M, controlled release with initial burst within 1 h, and sustained release for 48 h. The results of the animal test showed an appropriate concentration of FGF-7 for wound healing. In addition, 2-GHC-FD-8 is a suitable scaffold for wound healing. Therefore, we suggest that 2-GHC-FD-8/F3 is a useful wound dressing for accelerating wound healing.
