Aconitum lycoctonum L. (Ranunculaceae) mediated biogenic synthesis of silver nanoparticles as potential antioxidant, anti-inflammatory, antimicrobial and antidiabetic agents
Zia ur Rehman Khan,
Nasir Assad,
Muhammad Naeem-ul-Hassan,
Muhammad Sher,
Fatema Suliman Alatawi,
Mohsen Suliman Alatawi,
Awatif M. E. Omran,
Rasha M. A. Jame,
Muhammad Adnan,
Muhammad Nauman Khan,
Baber Ali,
Sana Wahab,
Sarah Abdul Razak,
Muhammad Ammar Javed,
Alevcan Kaplan,
Mehdi Rahimi
Affiliations
Zia ur Rehman Khan
Institute of Chemistry, University of Sargodha
Nasir Assad
Institute of Chemistry, University of Sargodha
Muhammad Naeem-ul-Hassan
Institute of Chemistry, University of Sargodha
Muhammad Sher
Institute of Chemistry, University of Sargodha
Fatema Suliman Alatawi
Department of Biochemistry, Faculty of Science, University of Tabuk
Mohsen Suliman Alatawi
Department of Pediatrics, College of Medicine, King Saud Bin Abdulaziz University for Health Sciences
Awatif M. E. Omran
Department of Biochemistry, Faculty of Science, University of Tabuk
Rasha M. A. Jame
Department of Chemistry, Faculty of Science, University of Tabuk
Muhammad Adnan
Department of Chemistry, Islamia College Peshawar
Muhammad Nauman Khan
Department of Botany, Islamia College Peshawar
Baber Ali
Department of Plant Sciences, Quaid-I-Azam University
Sana Wahab
Department of Plant Sciences, Quaid-I-Azam University
Sarah Abdul Razak
Institute of Biological Sciences, Faculty of Science, Universiti Malaya
Muhammad Ammar Javed
Institute of Industrial Biotechnology, Government College University
Alevcan Kaplan
Department of Crop and Animal Production, Sason Vocational School, Batman University
Mehdi Rahimi
Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology
Abstract In this study, a polar extract of Aconitum lycoctonum L. was used for the synthesis of silver nanoparticles (AgNPs), followed by their characterization using different techniques and evaluation of their potential as antioxidants, amylase inhibitors, anti-inflammatory and antibacterial agents. The formation of AgNPs was detected by a color change, from transparent to dark brown, within 15 min and a surface resonance peak at 460 nm in the UV–visible spectrum. The FTIR spectra confirmed the involvement of various biomolecules in the synthesis of AgNPs. The average diameter of these spherical AgNPs was 67 nm, as shown by the scanning electron micrograph. The inhibition zones showed that the synthesized nanoparticles inhibited the growth of Gram-positive and negative bacteria. FRAP and DPPH assays were used to demonstrate the antioxidant potential of AgNPs. The highest value of FRAP (50.47% AAE/mL) was detected at a concentration of 90 ppm and a DPPH scavenging activity of 69.63% GAE was detected at a concentration of 20 µg/mL of the synthesized AgNPs. 500 µg/mL of the synthesized AgNPs were quite efficient in causing 91.78% denaturation of ovalbumin. The AgNPs mediated by A. lycoctonum also showed an inhibitory effect on α-amylase. Therefore, AgNPs synthesized from A. lycoctonum may serve as potential candidates for antibacterial, antioxidant, anti-inflammatory, and antidiabetic agents.
