Identification of a Bioactive Compound against Adult T-cell Leukaemia from Bitter Gourd Seeds
Hisahiro Kai,
Ena Akamatsu,
Eri Torii,
Hiroko Kodama,
Chizuko Yukizaki,
Isao Akagi,
Hisatoshi Ino,
Yoichi Sakakibara,
Masahito Suiko,
Ikuo Yamamoto,
Akihiko Okayama,
Kazuhiro Morishita,
Hiroaki Kataoka,
Koji Matsuno
Affiliations
Hisahiro Kai
Department of Pharmaceutical Health Sciences, School of Pharmaceutical Sciences, Kyushu University of Health and Welfare, 1714-1 Yoshino-machi, Nobeoka, Miyazaki 882-8508, Japan
Ena Akamatsu
Research Promotion Bureau for Collaboration of Regional Entities, Miyazaki Prefectural Industrial Support Foundation, 16500-2 Higashi-Kaminaka, Sadowara-cho, Miyazaki, Miyazaki 880-0303, Japan
Eri Torii
Research Promotion Bureau for Collaboration of Regional Entities, Miyazaki Prefectural Industrial Support Foundation, 16500-2 Higashi-Kaminaka, Sadowara-cho, Miyazaki, Miyazaki 880-0303, Japan
Hiroko Kodama
Research Promotion Bureau for Collaboration of Regional Entities, Miyazaki Prefectural Industrial Support Foundation, 16500-2 Higashi-Kaminaka, Sadowara-cho, Miyazaki, Miyazaki 880-0303, Japan
Chizuko Yukizaki
Miyazaki Prefectural Food Research and Development Center, 16500-2 Higashi-Kaminaka, Sadowara-cho, Miyazaki, Miyazaki 880-0303, Japan
Isao Akagi
Research Promotion Bureau for Collaboration of Regional Entities, Miyazaki Prefectural Industrial Support Foundation, 16500-2 Higashi-Kaminaka, Sadowara-cho, Miyazaki, Miyazaki 880-0303, Japan
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki, Miyazaki 889-2192, Japan
Masahito Suiko
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki, Miyazaki 889-2192, Japan
Ikuo Yamamoto
Department of Rheumatology, Infectious Diseases and Laboratory Medicine, Faculty of Medicine, University of Miyazaki, 5200 Kihara Kiyotake, Miyazaki, Miyazaki 889-1692, Japan
Akihiko Okayama
Department of Rheumatology, Infectious Diseases and Laboratory Medicine, Faculty of Medicine, University of Miyazaki, 5200 Kihara Kiyotake, Miyazaki, Miyazaki 889-1692, Japan
Kazuhiro Morishita
Division of Tumor and Cellular Biochemistry, Department of Medical Sciences, Faculty of Medicine, University of Miyazaki, 5200 Kihara Kiyotake, Miyazaki, Miyazaki 889-1692, Japan
Hiroaki Kataoka
Section of Oncopathology and Regenerative Biology, Department of Pathology, Faculty of Medicine, University of Miyazaki, 5200 Kihara Kiyotake, Miyazaki, Miyazaki 889-1692, Japan
Koji Matsuno
Department of Pharmaceutical Health Sciences, School of Pharmaceutical Sciences, Kyushu University of Health and Welfare, 1714-1 Yoshino-machi, Nobeoka, Miyazaki 882-8508, Japan
In our previous report, an 80% ethanol bitter gourd seed extract (BGSE) was found to suppress proliferation of adult T-cell leukemia (ATL) cell lines. The present study aimed to identify the bioactive compounds from BGSE specific against ATL. From the result of an HPLC-MS analysis, α-eleostearic acid (α-ESA) was present in BGSE at 0.68% ± 0.0022% (±SD, n = 5). In the cell proliferation test, α-ESA potently suppressed proliferation of two ATL cell lines (ED and Su9T01; IC50 = 8.9 and 29.3 µM, respectively) more than several other octadecanoic acids. However, α-ESA moderately inhibited phytohemagglutinin-activated human peripheral blood mononuclear cells (PBMC; IC50 = 31.0 µM). These results suggest that BGSE-derived α-ESA has potential as a functional food constituent because of its activity against ATL, particularly against ED cells. Moreover, α-ESA might be effective for the prevention of moderate adverse effects of ATL on normal T cells.
