A review of tamoxifen administration regimen optimization for Cre/loxp system in mouse bone study

Abstract

Gene knockout is a technique routinely used in basic experimental research, particularly in mouse skeletal and developmental studies. Tamoxifen-induced Cre/loxp system is known for its temporal and spatial precision and commonly utilized by researchers. However, tamoxifen has been shown its side effects on affecting the phenotype of mouse bone directly. This review aimed to optimize tamoxifen administration regimens including its dosage and duration, to identify an optimal induction strategy that minimizes potential side effects while maintaining recombination efficacy. This study will help researchers in designing gene knockout experiments in bone when using tamoxifen.

Keywords

• Tamoxifen-induced side effects
• Tamoxifen administration
• Bone metabolism
• Cre/loxp system
• Mouse bone