Development of primary cell culture from the caudal fin region of snow trout Schizothorax plagiostomus (Heckel)

Abstract

A primary cell culture has been established from the Snow trout Schizothorax plagiostomus. Primary cultures have developed from caudal fin tissues by explant culture technique. Cell outgrowth has been obtained from the caudal fin explant after 5-7 days of explant culture. The culture medium used for the experiment was Leibovitz-15 supplemented with 20% Fetal Bovine Serum. Radiation of cells from the explants started after 2 to 3 days. Both fibroblast like and epithelial like cells were noticed but fibroblast like cells dominated as the growth progressed.

Keywords

• explant technique
• fetal bovine serum
• leibovitz-15media
• primary cell culture
• Schizothorax plagiostomus