In vitro propagation of Peperomia albovittata and Peperomia galioides by organogenesis

Abstract

Peperomia albovittata and P. galioides are two herbaceous species, belonging to the Piperaceae family, collected in the Andean district of Kañaris (1128 to 1550 m above sea level), in Lambayeque region, Peru. These species are recalcitrant to propagation by conventional vegetative methods, whereby the present study aims to in vitro propagate of Peperomia albovittata and P. galioides through by organogenesis. Single nodes with axillar buds, stem segments (internodes), leaves and petioles were cultured on gellified basal culture medium and was added IAA-GA3, NAA-GA3 and NAA-BAP at different concentrations. Only in the NAA-GA3 combination clonal propagation from nodal segments and direct organogenesis in internodes were observed. Callus induction and proliferation was observed in some interactions of NAA-BAP, although all explants from leaves turned dark brown and died. On the other hand, shoot regeneration was observed only in P. galioides. Both species, especially P. albovittata, showed a high degree of recalcitrance to the induction of in vitro morphogenic processes.

Keywords

• callus induction
• clonal propagation
• indirect organogenesis
• naa-ga3 combination
• vitrification and phenolic browning