Characterization of Powdery Mildew Resistance Donors within Triticum boeoticum Accessions using RAPDs

Abstract

Wide hybridization has become a technique suitable for introgression of alien genes into modern wheat cultivars. Ten Triticum boeoticum Boiss. accessions resistant to powdery mildew were studied in more detail. Variability between the accessions was studied using resistance tests, RAPD and storage protein analysis. Potential gene recipients (T. aestivum and T. durum cultivars) were also included. Unlike protein analysis we were able to clearly distinguish among the individual accessions of T. boeotium using RAPD. Computed dendrograms reflect phylogenetic relationships among the characterized species. All tests proved, that T. boeoticum is a valuable gene-pool. Three T. boeoticum × T. durum amphidiploid progenies were also analysed. They differ in the degree of resistance to powdery mildew. Both RAPD and storage protein analyses indicated that genome rearrangements occurred. We showed, that RAPD could be used to trace such events.

Keywords

• t. boeoticum
• wild wheat
• rapd
• genetic diversity
• resistance
• wide hybridization