Genetic Differentiation of an Endangered Megalobrama terminalis Population in the Heilong River within the Genus Megalobrama

Xuesong Hu; Bo Ma; Chitao Li; Zhiying Jia; Xiaona Jiang; Yanlong Ge; Jingou Tong; Lianyu Shi

doi:10.3390/d12100404

Diversity (Oct 2020)

Genetic Differentiation of an Endangered Megalobrama terminalis Population in the Heilong River within the Genus Megalobrama

Xuesong Hu,
Bo Ma,
Chitao Li,
Zhiying Jia,
Xiaona Jiang,
Yanlong Ge,
Jingou Tong,
Lianyu Shi

Affiliations

Xuesong Hu: National and Local United Engineering Laboratory for Freshwater Fish Breeding, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150076, China
Bo Ma: National and Local United Engineering Laboratory for Freshwater Fish Breeding, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150076, China
Chitao Li: National and Local United Engineering Laboratory for Freshwater Fish Breeding, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150076, China
Zhiying Jia: National and Local United Engineering Laboratory for Freshwater Fish Breeding, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150076, China
Xiaona Jiang: National and Local United Engineering Laboratory for Freshwater Fish Breeding, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150076, China
Yanlong Ge: National and Local United Engineering Laboratory for Freshwater Fish Breeding, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150076, China
Jingou Tong: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
Lianyu Shi: National and Local United Engineering Laboratory for Freshwater Fish Breeding, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150076, China

DOI: https://doi.org/10.3390/d12100404
Journal volume & issue: Vol. 12, no. 10
p. 404

Abstract

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Megalobrama terminalis, which inhabits the Sino-Russian Heilong-Amur River Basin, has decreased critically since the 1960s. It has been listed in the Red Book of Endangered Fish Species by the Russian Federation in 2004. To guide the utilization and conservation programs of M. terminalis in the Heilong River (MTH), 3.1 kb of mitochondrial DNA (mtDNA) concatenated sequences and sequence-related amplified polymorphism (SRAP) markers (15 primer combinations) were applied to explore the genetic divergence and population differentiation of MTH within the genus Megalobrama. Clear genetic divergence between MTH and six other populations of the genus Megalobrama was found by haplotype network (mtDNA) and principal component (SRAP) analyses. Moreover, the STRUCTURE analysis based on SRAP data showed that MTH could be assigned to a particular cluster, whereas conspecific M. terminalis in the Qiantang River and Jinsha River Reservoir belonged to the same cluster. Analysis of molecular variance (AMOVA) and Fst statistics for the mtDNA and SRAP data revealed significant genetic variance and differentiation among all detected populations. Taken together, the results suggest that MTH has a strong genetic differentiation from other populations within the genus Megalobrama, which contributes to effective utilization in artificial cultivation and breeding of MTH. Furthermore, these results also provide a scientific basis for the management of MTH as a separate conservation unit.

Published in Diversity

ISSN: 1424-2818 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General)
Website: http://www.mdpi.com/journal/diversity/

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