Open source fraction collector/MALDI spotter for proteomics
Scott B. Ficarro,
William Max Alexander,
Isidoro Tavares,
Jarrod A. Marto
Affiliations
Scott B. Ficarro
Department of Cancer Biology, Blais Proteomics Center, Dana-Farber Cancer Institute, Department of Pathology, Brigham and Women’s Hospital and Harvard Medical School, 360 Longwood Avenue, LC 2208, Boston, MA 02215-5450, USA
William Max Alexander
Department of Cancer Biology, Blais Proteomics Center, Dana-Farber Cancer Institute, Department of Pathology, Brigham and Women’s Hospital and Harvard Medical School, 360 Longwood Avenue, LC 2208, Boston, MA 02215-5450, USA
Isidoro Tavares
Department of Cancer Biology, Blais Proteomics Center, Dana-Farber Cancer Institute, Department of Pathology, Brigham and Women’s Hospital and Harvard Medical School, 360 Longwood Avenue, LC 2208, Boston, MA 02215-5450, USA
Jarrod A. Marto
Corresponding author.; Department of Cancer Biology, Blais Proteomics Center, Dana-Farber Cancer Institute, Department of Pathology, Brigham and Women’s Hospital and Harvard Medical School, 360 Longwood Avenue, LC 2208, Boston, MA 02215-5450, USA
We describe a complete open-source hardware/software solution for high performance thermostatted peptide fraction collection to support mass spectrometry experiments with complex proteomes. The instrument is easy to assemble using parts readily available through retail channels at a fraction of the cost compared to typical commercial systems. Control software is written in Python allowing for rapid customization. We demonstrate several useful applications, including the automated deposition of LC separated peptides for matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) as well as collection and concatenation of peptide fractions from nanoflow HPLC separations.
