The therapeutic effect of tanshinone IIA in mouse astrocytes after treatment with Angiostrongylus cantonensis fifth-stage larval excretory-secretory products

Kuang-Yao Chen; Yi-Ju Chen; Chien-Ju Cheng; Kai-Yuan Jhan; Cheng-Hsun Chiu; Lian-Chen Wang

Journal of Microbiology, Immunology and Infection (Aug 2023)

The therapeutic effect of tanshinone IIA in mouse astrocytes after treatment with Angiostrongylus cantonensis fifth-stage larval excretory-secretory products

Kuang-Yao Chen,
Yi-Ju Chen,
Chien-Ju Cheng,
Kai-Yuan Jhan,
Cheng-Hsun Chiu,
Lian-Chen Wang

Affiliations

Kuang-Yao Chen: Department of Parasitology, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan; Molecular Infectious Disease Research Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan; Department of Parasitology, School of Medicine, China Medical University, Taichung, 404, Taiwan; Corresponding author. Department of Parasitology, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan. Fax: +886 3 2218385.
Yi-Ju Chen: Department of Parasitology, School of Medicine, China Medical University, Taichung, 404, Taiwan
Chien-Ju Cheng: Department of Parasitology, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan
Kai-Yuan Jhan: Department of Parasitology, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan
Cheng-Hsun Chiu: Molecular Infectious Disease Research Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan
Lian-Chen Wang: Department of Parasitology, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan; Molecular Infectious Disease Research Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan; Corresponding author. Department of Parasitology, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan. Fax: +886 3 2218385.

Journal volume & issue: Vol. 56, no. 4
pp. 853 – 862

Abstract

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Background: Angiostrongylus cantonensis is an important food-borne zoonotic parasite that causes eosinophilic meningitis and meningoencephalitis in humans. Excretory-secretory products (ESPs) are valuable targets for studying host-parasite relationships. ESPs are composed of a variety of molecules that are used to penetrate defensive barriers and avoid immune attack of the host. Tanshinone IIA (TSIIA) is a vasoactive cardioprotective drug that is widely used in studies evaluating potential therapeutic mechanisms. In this study, we will evaluate the therapeutic effects of TSIIA in mouse astrocytes after A. cantonensis fifth-stage larvae (L5) ESPs treatment. Methods: Here, we examined the therapeutic effect of TSIIA by real-time qPCR, western blotting, activity assay, and cell viability assays. Results: First, the results showed that TSIIA can elevate cell viability in astrocytes after stimulation with ESPs. On the other hand, TSIIA downregulated the expression of apoptosis-related molecules. However, the expression of molecules related to antioxidant, autophagy, and endoplasmic reticulum stress was significantly increased. The results of antioxidant activation assays showed that the activities of superoxide dismutase (SOD), glutathione S-transferase (GST), and catalase were significantly increased. Finally, we found that cell apoptosis and oxidative stress were reduced in TSIIA-treated astrocytes by immunofluorescence staining. Conclusion: The findings from this study suggest that TSIIA can reduce cellular damage caused by A. cantonensis L5 ESPs in astrocytes and clarify the related molecular mechanisms.

Published in Journal of Microbiology, Immunology and Infection

ISSN: 1684-1182 (Print)
Publisher: Elsevier
Country of publisher: Hong Kong
LCC subjects: Science: Microbiology
Website: https://www.sciencedirect.com/journal/journal-of-microbiology-immunology-and-infection

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