Nature Communications (Sep 2018)

Mapping of histone-binding sites in histone replacement-completed spermatozoa

  • Keisuke Yoshida,
  • Masafumi Muratani,
  • Hiromitsu Araki,
  • Fumihito Miura,
  • Takehiro Suzuki,
  • Naoshi Dohmae,
  • Yuki Katou,
  • Katsuhiko Shirahige,
  • Takashi Ito,
  • Shunsuke Ishii

DOI
https://doi.org/10.1038/s41467-018-06243-9
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 11

Abstract

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While a majority of histones are replaced by protamines during spermatogenesis, a small amount is retained in mammalian spermatozoa. Here the authors develop a method to purify histones from replacement-completed sperm (HRCS), completely solubilize histones from cross-linked HRCS without MNase digestion, and map histone-binding sites in these cells.