Circulating Levels of Adipokines and TNF in Patients with and without Type 1 Diabetes

Abstract

Objective: The aim of this study was to evaluate circulating levels of adiponectin, resistin, visfatin and TNFin type1 diabetic children and young adolescents. Patients and Methods: Design: Cross-sectional study Setting: Ziauddin University Karachi Subjects: 90 subjects (30 type I diabetic (T1DM) subjects and 60 non-diabetic controls) between the age of05-15 years. Methods: Serum adiponectin, resistin, visfatin, TNF and insulin were measured using ELISA and fastingblood sugar by glucose oxidase (Standard Kit method). Results: Out of 90 study subjects, 30 were type 1 diabetic patients and 60 non-diabetic controls. Increased levelsof serum adiponectin levels were significant in type I diabetic subjects (TIDM) (10.83+1.73) compared withcontrols (9.07+1.25). Same pattern was observed for serum TNF in diabetic subjects (10.82+4.53) comparedwith controls (7.22+ 2.22). Significantly increased serum visfatin levels were found in TIDM patients (10.89+2.72)compared with controls (4.97+1.57). Significant negative correlation was observed between TNF and resistin(r=-0.356), adiponectin and age (r= - 0.594), and glucose and insulin in TIDM patients (r= -0.588). Binarylogistic shows significant association of HOMA IR, adiponectin and visfatin with Type 1 diabetes mellitus.Level of significance set for the study was P=0.05. Conclusion: Increased levels of circulating adipocytokines in Type I diabetic children depict the important roleof cytokines in Type I diabetic patients. However, increased adiponectin concentration in type I diabetic subjectsneeds to be further explored in large-scale studies

Keywords

• homa-ir
• adiponectin
• resistin
• visfatin
• tnf
• insulin.