Progress in application of nano-mimetic enzymes in reactive oxygen species detection

Abstract

Reactive oxygen species (ROS) as the substances with strong reactive activity, mainly include hydroxyl radicals (·OH), superoxide anion radicals (·O2-) and hydrogen peroxide (H2O2). Abnormal concentration of reactive oxygen species in mammals can cause serious diseases such as diabetes, cancer, tumors and some neurodegenerative diseases. Therefore, an increasing interest in the detection of ROS and issues was focused on and the consideration of the development of ROS detection sensors (e.g., biocompatibility, stability of the detection method, variability of different types of ROS, etc.) were raised. In the past, electrochemical sensors based on natural enzymes have been developed, but the drawbacks of natural enzymes such as complicated preparation, difficult purification, poor catalytic activity and stability, which limit their research and application. In recent years, numerous studies have found that nano-mimetic enzymes have unique enzyme-like properties such as catalytic activity, selectivity and stability, which can be widely used to construct electrochemical biosensors for the detection of reactive oxygen species. Recent nano-mimetic enzyme sensors for the detection of ·O2-, H2O2 and ·OH were focused in this paper, which are used in the field of life sciences to prevent related diseases in advance through the detection of reactive oxygen species, and can be used in the field of environmental pollution prevention and control for the quantitative detection of reactive oxygen species content.

Keywords

• reactive oxygen species
• nanomaterial
• nano-mimetic enzyme
• electrochemical detection
• sensor