iScience (Oct 2023)
Development of a multiassay algorithm (MAA) to identify recent HIV infection in newly diagnosed individuals in Indonesia
- Wahyu Nawang Wulan,
- Evy Yunihastuti,
- Dona Arlinda,
- Tuti Parwati Merati,
- Rudi Wisaksana,
- Dewi Lokida,
- Zehava Grossman,
- Kristi Huik,
- Chuen-Yen Lau,
- Nugroho Harry Susanto,
- Herman Kosasih,
- Abu Tholib Aman,
- Sunarto Ang,
- Rita Evalina,
- Anak Agung Ayu Yuli Gayatri,
- Chakrawati Hayuningsih,
- Agnes Rengga Indrati,
- July Kumalawati,
- Vivi Keumala Mutiawati,
- Mario Bernardinus Realino Nara,
- Asvin Nurulita,
- Rahmawati Rahmawati,
- Adria Rusli,
- Musofa Rusli,
- Dewi Yennita Sari,
- Justina Sembiring,
- Muchlis Achsan Udji Sofro,
- Wiwi Endang Susanti,
- Janice Tandraeliene,
- Fransisca Lianiwati Tanzil,
- Aaron Neal,
- Muhammad Karyana,
- Pratiwi Sudarmono,
- Frank Maldarelli
Affiliations
- Wahyu Nawang Wulan
- Doctoral Program in Biomedical Sciences, Faculty of Medicine Universitas Indonesia, Jakarta 10430, Indonesia; The Indonesia Research Partnership on Infectious Disease (INA-RESPOND), Jakarta 10560, Indonesia; HIV Dynamics and Replication Program, National Cancer Institute, Frederick, MD 21702, USA; Corresponding author
- Evy Yunihastuti
- Department of Internal Medicine, Faculty of Medicine Universitas Indonesia – HIV Integrated Clinic, Cipto Mangunkusumo Hospital, Jakarta 10430, Indonesia
- Dona Arlinda
- The Indonesia Research Partnership on Infectious Disease (INA-RESPOND), Jakarta 10560, Indonesia; Health Policy Agency, Ministry of Health Republic of Indonesia, Jakarta 10560, Indonesia
- Tuti Parwati Merati
- Sanglah Hospital, Denpasar 80113, Indonesia
- Rudi Wisaksana
- dr. Hasan Sadikin Hospital, Bandung 40161, Indonesia
- Dewi Lokida
- The Indonesia Research Partnership on Infectious Disease (INA-RESPOND), Jakarta 10560, Indonesia; Tangerang District Hospital, Tangerang 15111, Indonesia
- Zehava Grossman
- HIV Dynamics and Replication Program, National Cancer Institute, Frederick, MD 21702, USA; School of Public Health, Tel Aviv University, Tel Aviv 69978, Israel
- Kristi Huik
- HIV Dynamics and Replication Program, National Cancer Institute, Frederick, MD 21702, USA; Department of Microbiology, University of Tartu, 50090 Tartu, Estonia
- Chuen-Yen Lau
- HIV Dynamics and Replication Program, National Cancer Institute, Frederick, MD 21702, USA
- Nugroho Harry Susanto
- The Indonesia Research Partnership on Infectious Disease (INA-RESPOND), Jakarta 10560, Indonesia
- Herman Kosasih
- The Indonesia Research Partnership on Infectious Disease (INA-RESPOND), Jakarta 10560, Indonesia
- Abu Tholib Aman
- dr. Sardjito Hospital, Yogyakarta 55284, Indonesia
- Sunarto Ang
- A. Wahab Sjahranie Hospital, Samarinda 75123, Indonesia
- Rita Evalina
- H Adam Malik Hospital, Medan 20136, Indonesia
- Anak Agung Ayu Yuli Gayatri
- Sanglah Hospital, Denpasar 80113, Indonesia
- Chakrawati Hayuningsih
- Tangerang District Hospital, Tangerang 15111, Indonesia
- Agnes Rengga Indrati
- dr. Hasan Sadikin Hospital, Bandung 40161, Indonesia
- July Kumalawati
- dr. Cipto Mangunkusumo Hospital, Jakarta 10430, Indonesia
- Vivi Keumala Mutiawati
- dr. Zainoel Abidin Hospital, Banda Aceh 24415, Indonesia
- Mario Bernardinus Realino Nara
- dr. TC Hillers Hospital, Maumere 86111, Indonesia
- Asvin Nurulita
- dr. Wahidin Sudirohusodo Hospital, Makassar 90245, Indonesia
- Rahmawati Rahmawati
- dr. H. M. Ansari Saleh Hospital, Banjarmasin 70234, Indonesia
- Adria Rusli
- Prof. Dr. Sulianti Saroso Infectious Hospital, Jakarta 14340, Indonesia
- Musofa Rusli
- Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga / Dr. Soetomo Hospital, Surabaya 60286, Indonesia
- Dewi Yennita Sari
- Persahabatan Hospital, Jakarta 132030, Indonesia
- Justina Sembiring
- Abepura Hospital, Jayapura 99351, Indonesia
- Muchlis Achsan Udji Sofro
- dr. Kariadi Hospital, Semarang 50244, Indonesia
- Wiwi Endang Susanti
- dr. Soedarso Hospital, Pontianak 78124, Indonesia
- Janice Tandraeliene
- St. Carolus Hospital, Jakarta 10440, Indonesia
- Fransisca Lianiwati Tanzil
- Budi Kemuliaan Hospital, Batam 29483, Indonesia
- Aaron Neal
- Collaborative Clinical Research Branch, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA
- Muhammad Karyana
- The Indonesia Research Partnership on Infectious Disease (INA-RESPOND), Jakarta 10560, Indonesia; Health Policy Agency, Ministry of Health Republic of Indonesia, Jakarta 10560, Indonesia
- Pratiwi Sudarmono
- Department of Microbiology, Faculty of Medicine, Universitas Indonesia – Cipto Mangunkusumo Hospital, Jakarta 10430, Indonesia
- Frank Maldarelli
- HIV Dynamics and Replication Program, National Cancer Institute, Frederick, MD 21702, USA; Corresponding author
- Journal volume & issue
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Vol. 26,
no. 10
p. 107986
Abstract
Summary: Ongoing HIV transmission is a public health priority in Indonesia. We developed a new multiassay algorithm (MAA) to identify recent HIV infection. The MAA is a sequential decision tree based on multiple biomarkers, starting with CD4+ T cells >200/μL, followed by plasma viral load (pVL) > 1,000 copies/ml, avidity index (AI) 12-month) and a test set (N = 80) of newly diagnosed (≤1-month) antiretroviral (ARV) drug naive individuals. Ten of eighty (12 · 5%) newly diagnosed individuals were classified as recent infections. Drug resistance mutations (DRMs) against reverse transcriptase inhibitors were identified in two individuals: one infected with HIV subtype C (K219Q, V179T) and the other with CRF01_AE (V179D). Ongoing HIV transmission, including infections with DRMs, is substantial in Indonesia.
