Preparation of a 3D printable high-performance GelMA hydrogel loading with magnetic cobalt ferrite nanoparticles

Yiwan Shi; Yiwan Shi; Zhaozhen Wang; Zhaozhen Wang; Xinting Zhou; Chengxiong Lin; Chao Chen; Botao Gao; Weikang Xu; Xiaofei Zheng; Tingting Wu; Huajun Wang; Huajun Wang

doi:10.3389/fbioe.2023.1132192

Frontiers in Bioengineering and Biotechnology (Mar 2023)

Preparation of a 3D printable high-performance GelMA hydrogel loading with magnetic cobalt ferrite nanoparticles

Yiwan Shi,
Yiwan Shi,
Zhaozhen Wang,
Zhaozhen Wang,
Xinting Zhou,
Chengxiong Lin,
Chao Chen,
Botao Gao,
Weikang Xu,
Xiaofei Zheng,
Tingting Wu,
Huajun Wang,
Huajun Wang

Affiliations

Yiwan Shi: Department of Bone and Joint Surgery and Sports Medicine Center, The First Affiliated Hospital, Jinan University, Guangzhou, China
Yiwan Shi: National Engineering Research Center for Healthcare Devices, Guangdong Key Lab of Medical Electronic Instruments and Polymer Material Products, Institute of Biological And Medical Engineering, Guangdong Academy of Sciences, Guangzhou, China
Zhaozhen Wang: Department of Bone and Joint Surgery and Sports Medicine Center, The First Affiliated Hospital, Jinan University, Guangzhou, China
Zhaozhen Wang: National Engineering Research Center for Healthcare Devices, Guangdong Key Lab of Medical Electronic Instruments and Polymer Material Products, Institute of Biological And Medical Engineering, Guangdong Academy of Sciences, Guangzhou, China
Xinting Zhou: National Engineering Research Center for Healthcare Devices, Guangdong Key Lab of Medical Electronic Instruments and Polymer Material Products, Institute of Biological And Medical Engineering, Guangdong Academy of Sciences, Guangzhou, China
Chengxiong Lin: National Engineering Research Center for Healthcare Devices, Guangdong Key Lab of Medical Electronic Instruments and Polymer Material Products, Institute of Biological And Medical Engineering, Guangdong Academy of Sciences, Guangzhou, China
Chao Chen: Department of Orthopedics, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China
Botao Gao: National Engineering Research Center for Healthcare Devices, Guangdong Key Lab of Medical Electronic Instruments and Polymer Material Products, Institute of Biological And Medical Engineering, Guangdong Academy of Sciences, Guangzhou, China
Weikang Xu: National Engineering Research Center for Healthcare Devices, Guangdong Key Lab of Medical Electronic Instruments and Polymer Material Products, Institute of Biological And Medical Engineering, Guangdong Academy of Sciences, Guangzhou, China
Xiaofei Zheng: Department of Bone and Joint Surgery and Sports Medicine Center, The First Affiliated Hospital, Jinan University, Guangzhou, China
Tingting Wu: National Engineering Research Center for Healthcare Devices, Guangdong Key Lab of Medical Electronic Instruments and Polymer Material Products, Institute of Biological And Medical Engineering, Guangdong Academy of Sciences, Guangzhou, China
Huajun Wang: Department of Bone and Joint Surgery and Sports Medicine Center, The First Affiliated Hospital, Jinan University, Guangzhou, China
Huajun Wang: The Guangzhou Key Laboratory of Basic and Translational Research on Chronic Diseases, The First Affiliated Hospital, Jinan University, Guangzhou, China

DOI: https://doi.org/10.3389/fbioe.2023.1132192
Journal volume & issue: Vol. 11

Abstract

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Osteosarcoma remains a worldwide concern due to the poor effectiveness of available therapies in the clinic. Therefore, it is necessary to find a safe and effective therapy to realize the complete resection of osteosarcoma and reconstruction of the bone defect. Magnetic hyperthermia based on magnetic nanoparticles can kill tumor cells by raising the temperature without causing the side effects of conventional cancer treatments. This research aims to design a high-performance magnetic hydrogel composed of gelatin methacrylate and highly magnetic cobalt ferrite (CFO) nanoparticles for osteosarcoma treatment. Specifically, CFO is surface functionalized with methacrylate groups (MeCFO). The surface modified CFO has good biocompatibility and stable solution dispersion ability. Afterward, MeCFO nanoparticles are incorporated into GelMA to fabricate a three-dimensional (3D) printable MeCFO/GelMA magnetic hydrogel and then photocross-linked by UV radiation. MeCFO/GelMA hydrogel has high porosity and swelling ability, indicating that the hydrogel possesses more space and good hydrophily for cell survival. The rheological results showed that the hydrogel has shear thinning property, which is suitable as a bioprinting ink to produce desired structures by a 3D printer. Furthermore, 50 μg/mL MeCFO not only decreases the cell activity of osteosarcoma cells but also promotes the osteogenic differentiation of mBMSCs. The results of the CCK-8 assay and live/dead staining showed that MeCFO/GelMA hydrogel had good cytocompatibility. These results indicated that MeCFO/GelMA hydrogel with potential antitumor and bone reconstruction functions is a promising therapeutic strategy after osteosarcoma resection.

Published in Frontiers in Bioengineering and Biotechnology

ISSN: 2296-4185 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology
Website: http://www.frontiersin.org/bioengineering_and_biotechnology

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