Background: Complications of surgery are one of the main causes of death and disability worldwide. Considering the importance of patient safety and the necessity of auditing in operation rooms, therefore, the aim of this study was to evaluate the auditory of safe surgical principles in operating rooms of educational Hospital centers of Mazandaran University of Medical Sciences in 2018. Materials and Methods: This was a descriptive cross-sectional study conducted on 100 surgical operations rooms in general surgery, orthopedic, urology, cardiology, ophthalmology, plastic surgery, and ENT Hospitals centers of Mazandaran University of Medical Sciences in the year 2018 within a period of three months. Safe surgical checklist, which included the patient's safety standards in the surgery room were evaluated. Data were analyzed using SPSS software version 16. Results: The mean Safe surgical score in Razi Hospital was 26.41%. In Fatemeh Zahra Hospital, the mean score was 30.43%. In Abuali-sina Hospital, the total mean score was 34.83%. In Imam Khomeini Hospital, the mean score was 37.1%, and the mean Safe surgical score of Zare hospital was equal to 52.28%. Conclusion: Hospitals of Mazandaran University of Medical Sciences were overall in desirable to very desirable levels in terms of the standards for safe surgical principles in operating rooms.