The Use of Mesenchymal Stem Cells in the Complex Treatment of Kidney Tuberculosis (Experimental Study)
Alexander N. Muraviov,
Tatiana I. Vinogradova,
Anna N. Remezova,
Boris M. Ariel,
Anna A. Gorelova,
Nadezhda V. Orlova,
Natalia M. Yudintceva,
Diljara S. Esmedliaeva,
Marina E. Dyakova,
Marine Z. Dogonadze,
Natalia V. Zabolotnykh,
Irina A. Garapach,
Olga S. Maslak,
Yuri A. Kirillov,
Sergei E. Timofeev,
Yulia S. Krylova,
Petr K. Yablonskiy
Affiliations
Alexander N. Muraviov
Saint-Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Health of the Russian Federation, 191036 Saint-Petersburg, Russia
Tatiana I. Vinogradova
Saint-Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Health of the Russian Federation, 191036 Saint-Petersburg, Russia
Anna N. Remezova
Saint-Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Health of the Russian Federation, 191036 Saint-Petersburg, Russia
Boris M. Ariel
Saint-Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Health of the Russian Federation, 191036 Saint-Petersburg, Russia
Anna A. Gorelova
Saint-Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Health of the Russian Federation, 191036 Saint-Petersburg, Russia
Nadezhda V. Orlova
Saint-Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Health of the Russian Federation, 191036 Saint-Petersburg, Russia
Natalia M. Yudintceva
Institute of Cytology of Russian Academy of Sciences, Tikhoretsky 4, 194064 Saint-Petersburg, Russia
Diljara S. Esmedliaeva
Saint-Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Health of the Russian Federation, 191036 Saint-Petersburg, Russia
Marina E. Dyakova
Saint-Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Health of the Russian Federation, 191036 Saint-Petersburg, Russia
Marine Z. Dogonadze
Saint-Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Health of the Russian Federation, 191036 Saint-Petersburg, Russia
Natalia V. Zabolotnykh
Saint-Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Health of the Russian Federation, 191036 Saint-Petersburg, Russia
Irina A. Garapach
Saint-Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Health of the Russian Federation, 191036 Saint-Petersburg, Russia
Olga S. Maslak
Saint-Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Health of the Russian Federation, 191036 Saint-Petersburg, Russia
Yuri A. Kirillov
Avtsyn Research Institute of Human Morphology of Federal State Budgetary Scientific Institution “Petrovsky National Research Centre of Surgery”, 119991 Moscow, Russia
Sergei E. Timofeev
Moscow Multidisciplinary Clinical Center “Kommunarka” of the Moscow Health Department, 108814 Moscow, Russia
Yulia S. Krylova
Saint-Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Health of the Russian Federation, 191036 Saint-Petersburg, Russia
Petr K. Yablonskiy
Saint-Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Health of the Russian Federation, 191036 Saint-Petersburg, Russia
In recent years, the application of mesenchymal stem cells (MSCs) has been recognized as a promising method for treatment of different diseases associated with inflammation and sclerosis, which include nephrotuberculosis. The aim of our study is to investigate the effectiveness of MSCs in the complex therapy of experimental rabbit kidney tuberculosis and to evaluate the effect of cell therapy on the reparative processes. Methods: To simulate kidney tuberculosis, a suspension of the standard strain Mycobacterium tuberculosis H37Rv (106 CFU) was used, which was injected into the cortical layer of the lower pole parenchyma of the left kidney under ultrasound control in rabbits. Anti-tuberculosis therapy (aTBT) was started on the 18th day after infection. MSCs (5 × 107 cells) were transplanted intravenously after the start of aTBT. Results: 2.5 months after infection, all animals showed renal failure. Conducted aTBT significantly reduced the level of albumin, ceruloplasmin, elastase and the severity of disorders in the proteinase/inhibitor system and increased the productive nature of inflammation. A month after MSC transplantation, the level of inflammatory reaction activity proteins decreased, the area of specific and destructive inflammation in kidneys decreased and the formation of mature connective tissue was noted, which indicates the reparative reaction activation.
