Electrochemical DNA biosensor for the detection of human papillomavirus E6 gene inserted in recombinant plasmid

Abstract

In the current study, we describe a novel, simple, inexpensive, sensitive, specific, stable and label-free electrochemical DNA biosensor used to identify a target gene cloned into a plasmid. The biosensor was designed with a 23-mer oligonucleotide of guanine-free, which was immobilized on the pencil graphite electrode (PGE) for E6 gene detection from human papillomavirus 16 type (HPV16). The E6 gene was used due to its clinical importance. The optimal probe concentration was obtained in 500 nM. The hybridization detection showed a good linearity in the range of 40–5,000 pg/μL with a detection limit of 16 pg/μL. The electrochemical method showed higher sensitivity and specificity when compared with the agarose gel electrophoresis assay. This technology could be postulated as a new and attractive alternative for cloning analysis in plasmids.

Keywords

• Electrochemical DNA biosensor
• Direct DNA detection
• Inosine-probe
• Human papillomavirus 16
• Electrophoresis standard method