Overlapping roles of spliceosomal components SF3B1 and PHF5A in rice splicing regulation

Haroon Butt; Jeremie Bazin; Sahar Alshareef; Ayman Eid; Moussa Benhamed; Anireddy S. N. Reddy; Martin Crespi; Magdy M. Mahfouz

doi:10.1038/s42003-021-02051-y

Communications Biology (May 2021)

Overlapping roles of spliceosomal components SF3B1 and PHF5A in rice splicing regulation

Haroon Butt,
Jeremie Bazin,
Sahar Alshareef,
Ayman Eid,
Moussa Benhamed,
Anireddy S. N. Reddy,
Martin Crespi,
Magdy M. Mahfouz

Affiliations

Haroon Butt: Laboratory for Genome Engineering and Synthetic Biology, King Abdullah, University of Science and Technology (KAUST)
Jeremie Bazin: CNRS, INRA, Institute of Plant Sciences Paris-Saclay IPS2, Univ Paris Sud, Univ Evry, Univ Paris-Diderot, Sorbonne Paris-Cite, Universite Paris-Saclay
Sahar Alshareef: Laboratory for Genome Engineering and Synthetic Biology, King Abdullah, University of Science and Technology (KAUST)
Ayman Eid: Laboratory for Genome Engineering and Synthetic Biology, King Abdullah, University of Science and Technology (KAUST)
Moussa Benhamed: CNRS, INRA, Institute of Plant Sciences Paris-Saclay IPS2, Univ Paris Sud, Univ Evry, Univ Paris-Diderot, Sorbonne Paris-Cite, Universite Paris-Saclay
Anireddy S. N. Reddy: Department of Biology and Program in Cell and Molecular Biology, Colorado State University
Martin Crespi: CNRS, INRA, Institute of Plant Sciences Paris-Saclay IPS2, Univ Paris Sud, Univ Evry, Univ Paris-Diderot, Sorbonne Paris-Cite, Universite Paris-Saclay
Magdy M. Mahfouz: Laboratory for Genome Engineering and Synthetic Biology, King Abdullah, University of Science and Technology (KAUST)

DOI: https://doi.org/10.1038/s42003-021-02051-y
Journal volume & issue: Vol. 4, no. 1
pp. 1 – 14

Abstract

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Butt et al. used CRISPR-mediated directed evolution to generate rice mutants for the spliceosome components SF3B1 and PHF5A. They demonstrate that these mutants have different levels of sensitivity to salt treatments and suggest that the strategies they employed can be used in the future to study functions of redundant paralogs in plants.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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