Journal of Pharmacy and Bioallied Sciences (Dec 2009)

Statistical Optimization of Process Parameters Influencing Biotransformation of Plant Tannin into Gallic Acid under Solid–Liquid Fermentation

  • Rupa Mazumder,
  • Rupa Mazumder,
  • Bibhu Prasad Panda,
  • Rintu Banerjee

Journal volume & issue
Vol. 1, no. 1
pp. 41 – 49

Abstract

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<p class="MsoNormal" style="text-align: justify; line-height: 200%;"><span style="color: black;">Gallic acid </span><span style="line-height: 200%; color: black;">(3, 4, 5-trihydroxy benzoic acid), an important organic acid for synthesis of </span><span style="color: black;">propyl gallate and trimethoprim, was produced by solid-liquid fermentation using <em>Rhizopus oryzae</em> NRRL 21498. Evolutionary operation (EVOP)-factorial design and response surface methodology was applied successfully to elucidate the effect of different process parameters influencing biotransformation of natural tannin (powdered chebulic myrobalan fruit) to gallic acid. At 28<sup>O</sup>C, 70% relative humidity, pH 6, 72h of fermentation period, 3ml inoculum volume and 25g substrate weight resulted maximum gallic acid yield of 93.29%.</span></p>

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