Efficacy of ozonated water, 2% chlorhexidine and 5.25% sodium hypochlorite on five microorganisms of endodontic infection: In vitro study

Abstract

Introduction: The aim of this study was to assess the antimicrobial efficacy of ozonated water (4 mg/l), 2% chlorhexidine solution, 5.25% sodium hypochlorite solution on five common endodontic microorganisms. Methods: The organisms chosen in the study were Enterococcus faecalis, Streptococcus mutans, Staphylococcus aureus, Candida albicans, Kocuria rhizophila. Agar well diffusion test direct contact test were used as methods to assess antimicrobial effectiveness. In agar well diffusion test, the maximum zone of inhibition formed around the well in an agar plate after incubation of test materials against each microorganism for 24 h and 48 h were measured. In direct contact test, the colonies of E. faecalis formed on agar plates with each test material were calculated. Results: The results showed that 2% chlorhexidine showed highest zone size and minimum colony forming units indicating its highest potency and ozonated water was showed the least efficacy with a significant difference between both groups. The colony forming units showed an increase in number when ozonated water was used against E. faecalis

Keywords

• 2% chlorhexidine
• 5.25% sodium hypochlorite
• agar well diffusion test
• antimicrobial efficacy
• Candida albicans
• colony forming units
• Enterococcus faecalis
• irrigation
• Kocuria rhizophila
• ozonated water
• Staphylococcus aureus
• Streptococcus mutans