Localization data of the T-DNA insertion site in Arabidopsis line SALK_146824C

Abstract

The present work reports on the determination of localization of T-DNA insertion in Arabidopsis thaliana SALK_146824C line. The line is characterized as deficient in GDP-l-galactose phosphorylase 1 (VTC2) gene encodes a protein involved in ascorbate biosynthesis. Primer pairs allow to distinguishing SALK_146824C from wild type plants and to the exact localization of the insertion were designed used. Sanger sequencing confirmed the location of the T-DNA insertion in the sixth exon of the gene. RT-PCR data shows nearly undetectable levels of VTC2 mRNA expression level in SALK_146824C line as compared with wild type plants of Columbia-0 line. Localization of the primer pair upstream and downstream the T-DNA insertion did not affect the expression values.

Keywords

• Arabidopsis thaliana
• T-DNA
• Mutant line
• VTC2