Kinetics Of The Enzymatic Transesterification Of Tuna Oil Catalyzed By Immobilized Candida Rugose Lipase To Produce Structured Lipid High In Omega-3 Fatty Acids

Abstract

Structured lipid containing Medium Chain of Fatty Acid (MCFA) at outer position and Poly-Unsaturated Fatty Acids (PUFA) at sn-2 position has nutritional value and excellent absorption. In this research, structured lipids was synthesized directly through enzymatic acidolysis between fish oil and lauric acid and catalyzed by specific lipase from immobilized 1.3 Candida rugose. The kinetics of enzymatic transesterification reactions catalyzed by immobilized Candida rugose was studied. To obtain the optimal condition, the factor substrate ratio of fish oil : lauric acid and reaction time were investigated. Simple mathematical model for DAG synthesis through transesterification mechanisms have been developed. The results showed that the parameters obtained had a good sensitivity. It was found that the kinetic model well describes the behavior of the reaction as the influence of the initial ratio of reactants.

Keywords

• kinetics
• MCFA
• catalyst
• Candida rugose
• tuna oil