Macroporous silicon-wollastonite scaffold with Sr/Se/Zn/Mg-substituted hydroxyapatite/chitosan hydrogel
Antonia Ressler,
Nikhil Kamboj,
Maja Ledinski,
Anamarija Rogina,
Inga Urlić,
Irina Hussainova,
Hrvoje Ivanković,
Marica Ivanković
Affiliations
Antonia Ressler
Faculty of Chemical Engineering and Technology, University of Zagreb, HR-10000, Zagreb, Marulićev trg 19, Croatia; Corresponding author. Faculty of Chemical Engineering and Technology, University of Zagreb, HR-10000, Zagreb, Marulićev trg 19, p.p.177, Croatia.
Nikhil Kamboj
Tallinn University of Technology, Department of Mechanical and Industrial Engineering, Ehitajate 5, 19086, Tallinn, Estonia; Turku Clinical Biomaterials Centre, Department of Biomaterials Science, Faculty of Medicine, Institute of Dentistry, University of Turku, FI-20014, Turku, Finland
Maja Ledinski
Faculty of Science, University of Zagreb, Horvatovac 102a, Zagreb, 10 000, Croatia
Anamarija Rogina
Faculty of Chemical Engineering and Technology, University of Zagreb, HR-10000, Zagreb, Marulićev trg 19, Croatia
Inga Urlić
Faculty of Science, University of Zagreb, Horvatovac 102a, Zagreb, 10 000, Croatia
Irina Hussainova
Tallinn University of Technology, Department of Mechanical and Industrial Engineering, Ehitajate 5, 19086, Tallinn, Estonia
Hrvoje Ivanković
Faculty of Chemical Engineering and Technology, University of Zagreb, HR-10000, Zagreb, Marulićev trg 19, Croatia
Marica Ivanković
Faculty of Chemical Engineering and Technology, University of Zagreb, HR-10000, Zagreb, Marulićev trg 19, Croatia
The scaffolds, which morphologically and physiologically mimic natural features of the bone, are of a high demand for regenerative medicine. To address this challenge, bioactive porous silicon/wollastonite (SC) scaffold has been developed for potential bone tissue engineering applications. Additive manufacturing through the selective laser melting approach has been exploited to fabricate computer-aided designed scaffolds with a pore size of 400 μm. To increase the biocompatibility and osteogenic properties of SC scaffolds, the hydrogel based on a mixture of four mono-substituted hydroxyapatites (sHAp) and biopolymer chitosan (CHT) has been incorporated into SC by impregnation and freeze-gelation method. The pore size of 400 μm of SC has provided enough space for the impregnation of polymer solution and composite (CHT/sHAp) suspension to form highly porous hydrogel within pores. By the combination of SC and CHT/sHAp, both cell attachment and homogeneous proliferation on SC scaffold as well as mechanical properties of CHT/sHAp hydrogel have been improved.
