Invariant surface glycoprotein 65 of Trypanosoma brucei is a complement C3 receptor

Olivia J. S. Macleod; Alexander D. Cook; Helena Webb; Mandy Crow; Roisin Burns; Maria Redpath; Stefanie Seisenberger; Camilla E. Trevor; Lori Peacock; Angela Schwede; Nicola Kimblin; Amanda F. Francisco; Julia Pepperl; Steve Rust; Paul Voorheis; Wendy Gibson; Martin C. Taylor; Matthew K. Higgins; Mark Carrington

doi:10.1038/s41467-022-32728-9

Nature Communications (Aug 2022)

Invariant surface glycoprotein 65 of Trypanosoma brucei is a complement C3 receptor

Olivia J. S. Macleod,
Alexander D. Cook,
Helena Webb,
Mandy Crow,
Roisin Burns,
Maria Redpath,
Stefanie Seisenberger,
Camilla E. Trevor,
Lori Peacock,
Angela Schwede,
Nicola Kimblin,
Amanda F. Francisco,
Julia Pepperl,
Steve Rust,
Paul Voorheis,
Wendy Gibson,
Martin C. Taylor,
Matthew K. Higgins,
Mark Carrington

Affiliations

Olivia J. S. Macleod: Department of Biochemistry, University of Cambridge
Alexander D. Cook: Department of Biochemistry, University of Oxford
Helena Webb: Department of Biochemistry, University of Cambridge
Mandy Crow: Department of Biochemistry, University of Cambridge
Roisin Burns: Department of Biochemistry, University of Cambridge
Maria Redpath: Department of Biochemistry, University of Cambridge
Stefanie Seisenberger: Department of Biochemistry, University of Cambridge
Camilla E. Trevor: Department of Biochemistry, University of Cambridge
Lori Peacock: Bristol Veterinary School and School of Biological Sciences, University of Bristol
Angela Schwede: Department of Biochemistry, University of Cambridge
Nicola Kimblin: Department of Biochemistry, University of Cambridge
Amanda F. Francisco: Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine
Julia Pepperl: Department of Biochemistry, University of Cambridge
Steve Rust: Antibody Discovery and Protein Engineering, Biopharmaceuticals R&D, AstraZeneca
Paul Voorheis: School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin
Wendy Gibson: Bristol Veterinary School and School of Biological Sciences, University of Bristol
Martin C. Taylor: Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine
Matthew K. Higgins: Department of Biochemistry, University of Oxford
Mark Carrington: Department of Biochemistry, University of Cambridge

DOI: https://doi.org/10.1038/s41467-022-32728-9
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 10

Abstract

Read online

Trypanosomes evade the immune response through antigenic variation of a surface coat containing variant surface glycoproteins (VSG). They also express invariant surface glycoproteins (ISGs), which are less well understood. Here, Macleod et al. show that ISG65 of T. brucei is a receptor for complement component 3. They provide the crystal structure of T. brucei ISG65 in complex with complement C3d and show evidence that ISG65 is involved in reducing trypanosome susceptibility to C3-mediated clearance in vivo.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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